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Molecular Motors

Molecular Motors. Move lipid dropletsHave two ?heads"Walk along microtubulesTake steps, like humansStudy kinesin and dynein. Kinesin vs. Dynein. Well knownTransports lipid droplets to positive end of nucleiHas a step size of 8nm. Harder to study because of speedTransports lipid drople

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Molecular Motors

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    1. Molecular Motors By Simone Siegel and Avery Baker (Dr. Shubeita)

    2. Molecular Motors Move lipid droplets Have two heads Walk along microtubules Take steps, like humans Study kinesin and dynein

    3. Kinesin vs. Dynein Well known Transports lipid droplets to positive end of nuclei Has a step size of 8nm Harder to study because of speed Transports lipid droplets to negative end of nuclei Has an unknown step size

    4. Overall Goal: slow down dynein and measure its step size It is easier to see individual steps when dynein is slowed down Study at colder temps. In vivo (inside the cell) Reducing the temp. breaks ATP bonds less often, less energy released, should move slower

    5. Procedure: fly embryos Learned fruit fly embryo preparation techniques (staging, peeling, mounting)

    6. Obtaining Data Record videos on cold DIC (Differential Interface Contrast) microscope

    8. Analyzing With video, position of lipid droplets can be tracked Excel spreadsheet used to graph the data from the lipid droplet tracked Data shows the speed of the lipid droplet

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