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Effective Scouting and Sample Submission for Soybean Rust Detection in Wisconsin

This guide provides essential information on scouting for soybean rust pathogens, including Phakopsora pachyrhizi and Phakopsora meibomiae, particularly under favorable weather conditions like high humidity and moderate temperatures. It outlines potential host plants such as soybean, pea, and various clovers. Effective scouting tips emphasize focusing on known hosts and thorough examination of leaves, including those in the lower canopy. Details on sample collection, packaging, and submission protocols to the Plant Disease Diagnostic Clinic (PDDC) are also included for accurate diagnostics.

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Effective Scouting and Sample Submission for Soybean Rust Detection in Wisconsin

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  1. Soybean RustScouting and Sample Submission • Pathogens: • Phakopsora pachyrhizi • Phakopsora meibomiae • Favorable environment • Long periods of leaf wetness • Moderate temperatures (59-77°F) • High relative humidity (75-80%)

  2. Soybean RustScouting and Sample Submission • Hosts potentially found in Wisconsin: • Soybean (Glycine max) • Snap/kidney bean (Phaseolus vulgaris) • Pea (Pisum sativum) • Yellow sweetclover (Melilotus officinalis) • White clover (Trifolium incarnatum) • Purple crownvetch (Cornilla varia) • Winter vetch (Vicia villosa)

  3. Soybean RustScouting and Sample Submission • Hosts potentially found in Wisconsin: • Lupine (Lupinus spp.) • Ticktrefoil (Desmodium spp.) • American bird’s-foot trefoil (Lotus unifoliolatus) • Crimson clover (Trifolium incarnatum) • Korean clover (Kummerowia stipulacea) • Chinese lespedeza (Lespedeza cuneata) • Rattlebox (Crotalaria spp.)

  4. Soybean RustScouting and Sample Submission • Tips for scouting • Concentrate efforts on known hosts (particularly soybean), but be willing to check other legumes for the disease. • Check leaves in the lower canopy first, but be aware that upper leaves can become infected too. • The more leaves you examine, the more likely you will find the disease.

  5. Soybean RustScouting and Sample Submission • Sample collection and submission • See UW-Extension Pest Alert XGT1001 for details. • DO NOT be bashful about sending in samples. • Submit a representative set of leaves (or other plant parts) showing a range of symptoms. • Collect and submit detailed information on the location from which the sample was collected. • Sandwich the plant materials between paper towels and cardboard.

  6. Soybean RustScouting and Sample Submission • Sample collection and submission • Double bag the sample. • Keep the sample cool prior to shipping. • Ship the sample to the PDDC by overnight mail if possible in a sturdy, leak-proof box. • Include a completed copy of the “Soybean Rust Submission Form”. • If possible, call the PDDC to let staff know that a sample is on the way.

  7. Soybean RustScouting and Sample Submission • What to expect • Samples will be processed as soon as they arrive. • Microscopic examination • Moist chamber incubation • PDDC staff will contact you by phone if the sample appears to be positive for soybean rust (confirmation from APHIS will be needed for a first report on any given host).

  8. Soybean RustScouting and Sample Submission • What to expect • PDDC staff will follow up with a written report detailing disease problems whether the sample is positive for soybean rust or not. • There will be no charge for soybean rust diagnostics.

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