10 likes | 104 Vues
This study investigates the relative expression of hoxd4 with miR-10a and miR-10b in MCF7 and MDA-MB-231 cell lines. The role of siRNAs and Ago proteins in regulating hox genes is also explored.
E N D
MCF7 MDA- MB-231 siP1 f Relative hoxd4 expression pri-miR-10b 1.5 1.2 siP2 0.9 0.6 * d * * 0.3 miR- 10a Cont siP2 siP1 0 hoxd4 Cont siAgo1 siAgo4 siAgo2 siAgo3 siDicer β-actin miR-10a miR-10a e a miR-10a sinc34 sinc35 Relative expression of hoxb/d4 sinc36 Cont 8 Cont siD1 siD2 miR-10a miR-10b 6 hoxd4 β-actin 4 sinc34 sinc35 sinc36 * Cont 2 b ** ** * 0 nc-hoxd4-34 nc-hoxd4-35 MDA- MB-231 MCF7 nc-hoxd4-36 β-actin hoxd4 hoxb4 c siP2 siP1