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Explore Mendel's principles in transmission genetics, solving sample problems to understand segregation. Analyze DNA fragments cut by PstI enzyme. Learn about monohybrid crosses and phenotypic ratios.
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Lecture 2:Jan. 23, 2006 Transmission genetics: Principle of segregation
Sample problem 5’-ATGCCCTGCAGTACCATGACGCGTTACGCAGCTGATCGAAACGCGTATATATGCC-3’ 3’-TACGGGACGTCATGGTACTGCGCAATGCGTCGACTAGCTTTGCGCATATATACGG-5’ The restriction site for the enzymes PstI is: 5’-CTGCAG-3’ If the enzyme PstI was used to cut the above DNA, what fragments would result?
Explanation of the 3:1 phenotypic ratio of dominant: recessive