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40 mM citrate, 100 nM Ca 2+ , 0.4 mM Mg 2+ PowerPoint Presentation
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40 mM citrate, 100 nM Ca 2+ , 0.4 mM Mg 2+

40 mM citrate, 100 nM Ca 2+ , 0.4 mM Mg 2+

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40 mM citrate, 100 nM Ca 2+ , 0.4 mM Mg 2+

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  1. Glut 40 mM citrate, 100 nM Ca2+, 0.4 mM Mg2+ Glut 1 min exposure (not 40 s) 051905a_s012

  2. 40 mM citrate, 100 nM Ca2+, 0.4 mM Mg2+ Glut Glut 051905a_s015-024

  3. Glut 40 mM citrate, 100 nM Ca2+, 0.4 mM Mg2+ EGTA 40 mM citrate causes a continuous, linear increase in [Ca2+]SR, to levels unattainable in anions that do not pass the SR membrane. No Ca2+ surge is apparent upon substitution of citrate for 50 mM EGTA (0 Ca). Note that this preparation was, following the above expt, exposed to glut, SO42- and then EGTA. The procedure saw a drop in [Ca2+]SR in SO42- and a Ca2+ surge upon substitution of SO42- for EGTA. 051905b