1 / 10

DNA Fingerprinting

DNA Fingerprinting. DNA forensic. Adalah metode analisis kejahatan forensik dengan menggunakan sampel DNA Contoh kasus : Bom Bali, JW Mariot , pembuktian jasad Nurdin M Top. Koleksi Spesimen Sampel. Sumber dapat diambil dr : - pakaian - rekatan amplop - putung rokok

nau
Télécharger la présentation

DNA Fingerprinting

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. DNA Fingerprinting

  2. DNA forensic • Adalahmetodeanalisiskejahatanforensikdenganmenggunakansampel DNA • Contohkasus: Bom Bali, JW Mariot, pembuktianjasadNurdin M Top

  3. KoleksiSpesimenSampel • Sumberdapatdiambildr: - pakaian - rekatanamplop - putungrokok - tepicangkir • Sampelygdiambildptberupa: darah, keringat, salivacairan semen, rambut, kuku, otot,dll

  4. Preparasisampel • Gunakansarungtangan disposable • Gunakaninstrumen disposable • Hindariberbicara, bersin, danbatuk pd saatpengambilansampel • Hidarimemegangbarangbukti • Pengeringansebelumpembungkusanbarangbukti • Hindarkandrsuhupanasdancahayamataharilangsung • Ditambahkanagen anti clotting (EDTA)M

  5. Ekstrak DNA • Dimurnikan dg larutan detergent (SDS/ fenol chloroform) untukmenghilangkan material selygtidakdiinginkan • Pengambilan DNA daridalamsel • Disentrifugasi (pemisahan)

  6. Analisis RFLP • Restriction Fragment Length Polymorphism (RFLP) • Ditambahkanenzimendonukleaserestriksiuntukmemotong DNA target pd sekuenstertentu. • Di elektroforesisuntukmemisahkanpotongan fragment DNA • Potongan DNA denganberatmolekulkecilakanlebihcepatbermigrasi (bp)

  7. Southern Blot • Setelahdielektroforesisfragmen pd gel agaroseditransferkemembrannitroselulosa • Cetakan pd nitroselulosa “fingerprint” • Diinkubasidenganditambahkanfragmen DNA radioaktif (probe) ygakanberhibridisasi dg sampel • Probe ygtidakberhibridisasiakandicucidihilangkan • Dilihatdibawahsinar X (autordiograph)

  8. PCR • Untukmengamplifikasi DNA sampelygjumlahnyasedikit. • DigunakanTaq Polymerase untukmembangunnukleotidabaru • Alat: thermal cycler

  9. VNTRs Variable Number Tandenm Repeats (VNTRs) Deretanbasaberulangpadaintron. VNTRs diwariskandrorangtua, ataukombinasidarikeduaorangtua. Biasadipakaiuntukidentifikasianggotakeluarga

  10. DNA mitokondria • Untukidentifikasihubunganantaraanakkandung – ibukandung • Me “match” kansidikjari DNA mitokondriadariduasampel

More Related