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Understanding the Activity of Chloroplasts

Understanding the Activity of Chloroplasts. AP Biology Core Lab # 9. DPIP (Blue). DPIP (Colorless). Measure color change (via transmittance) in spec @ 605 nm. Experimental Conditions. #1) Blank: Chloroplasts + Light -DPIP #2) Unboiled Chloroplasts – Light + DPIP

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Understanding the Activity of Chloroplasts

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  1. Understanding the Activity of Chloroplasts AP Biology Core Lab # 9

  2. DPIP (Blue) DPIP (Colorless) Measure color change (via transmittance) in spec @ 605 nm

  3. Experimental Conditions • #1) Blank: Chloroplasts + Light -DPIP • #2) Unboiled Chloroplasts – Light + DPIP • #3) Unboiled Chloroplasts + Light + DPIP • #4) Boiled Chloroplasts + Light + DPIP • #5) Control: No Chloroplasts + DPIP WHAT SHOULD HAPPEN TO TRANSMITTANCE IN ALL OF THESE CONDITIONS?

  4. The Data • Click for Excel Spreadsheet

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