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Microarray Hybridization Pattern of P. aeruginosa Outbreak Clone EC29

Explore the hybridization patterns of P. aeruginosa outbreak clone EC29 isolated from patient RS11 and the localization of marker genes on the typing chip. The Clondiag microarray depicts SNPs from core genome loci, converting alleles into hexadecimal codes for precise typing.

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Microarray Hybridization Pattern of P. aeruginosa Outbreak Clone EC29

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  1. Microarray hybridization pattern of the P. aeruginosa outbreak clone EC29 Microarray hybridization pattern of the P. aeruginosaoutbreak clone EC29 (#11) isolated from patient RS11 (A) and localization of marker genes on the typing chip (B). Only the lower part of the Clondiag microarray representing different single-nucleotide polymorphisms (SNPs) from seven conserved loci of the core genome of P. aeruginosais shown. For each SNP, the presence of PAO1-type (0) and non-PAO1-type (1) alleles is converted into a four-digit hexadecimal code as described in the materials and methods section. For the flagellin gene fliC only one allele is sufficient for typing (fliCa=1, then fliCb=0 and vice versa). Individual P. aeruginosaisolates carry either exoS gene or exoU gene (exoS=1, then exoU=0 and vice versa).

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