1 / 1

Analysis of DMF-Modified FKBP Digest Peptides Using ProZap C18 Chromatography

This study investigates the influence of DMF (Dimethylformamide) modification on FKBP (FK506 binding protein) digest peptides. We utilized ProZap C18 chromatography to analyze peptide retention times under various deuterium levels. The results revealed significant differences in retention times, averaging 18% higher deuterium levels due to DMF modification. Our analysis included fragmentation patterns represented as [M+H]+ and [M+2H]2+, identifying numerous common fragments (38) across varying retention times. This research contributes to understanding peptide behavior in modified conditions crucial for therapeutic applications.

neylan
Télécharger la présentation

Analysis of DMF-Modified FKBP Digest Peptides Using ProZap C18 Chromatography

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. DMF-Modified FKBP Digest Peptides: ProZap C18 Fig. S4 Conventional 2 6 RT = 2.3 min RT = 2.2 min 5 1.5 RT = 1.7 min 4 RT = 1.8 min Deuterium Level 1 3 2 0.5 1 [74-80][M+H]+ [26-36][M+2H]2+ 0 0 0 30 60 90 120 0 30 60 90 120 7 2.5 RT = 0.9 min RT = 0.8 min 6 2 5 RT = 1.7 min Deuterium Level 1.5 4 RT = 1.8 min 3 1 2 0.5 1 [37-49][M+2H]2+ [55-59][M+H]+ 0 0 0 30 60 90 120 0 30 60 90 120 Time (min) Average 18% Higher D Level with DMF Modifier (38 Common Fragments)

More Related