1 / 6

Mycobacterium tuberculosis

Mycobacterium tuberculosis. One third of the world population is currently infected. Over 8 million new cases of TB in 2007. 5-10% of those infected become sick or infectious at some time in their lives. An estimated 1.6 million TB deaths occurred in 2007 . Drug-resistance is on the rise

nuwa
Télécharger la présentation

Mycobacterium tuberculosis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mycobacterium tuberculosis • One third of the world population is currently infected. • Over 8 million new cases of TB in 2007. • 5-10% of those infected become sick or infectious at some time in their lives. • An estimated 1.6 million TB deaths occurred in 2007. • Drug-resistance is on the rise • No effective vaccine against pulmonary TB www.who.int

  2. Lines of Investigation– Schorey laboratory • Molecular and cellular basis of TB pathogenesis. • Exosomes as TB vaccine candidates • Diagnostic tools for TB. Supported by: National Institutes of Health - R01 AI056979 - R01 AI052438 Gates Exploration Grant

  3. Diagnostic needs: High specificity and sensitivity as well as a test which is rapid, requires minimum manipulation, easy to use and is of minimal cost. • What is available: • Acid-fast smear test using sputum • Culturing approaches using sputum as the source of bacilli • Detection from patient blood antibodies or T cells specific for • mycobacterial components • Detection of M.tb DNA or RNA in patient bodily fluids or sputum • Detection of other mycobacterial components in patient bodily fluids

  4. TB Diagnostic Development Through Research and Industrial Collaboration • Indiana Clinical and Translational Sciences Institute (ICTSI) • University of Notre Dame’s College of Engineering and College of Science • Advanced Diagnostics and Therapeutics and the Eck Institute for Global • Health • Academic Model for the Prevention and Treatment of HIV/AIDS (AMPATH) • and the IU School of Medicine • Companion Diagnostics Inc. • Sony DADC (Polymer Devices Branch of SONY)

  5. TB Detection Project Isolation of M. tuberculosis Lipoarabinomannan (LAM) from urine of TB patients using an antibody-based microfluidic detection platform • Couple LAM antibodies to chip/sensor substrate using • micro-stamping techniques. • Converting current glass microfluidic platform to hard plastic, • including synthesis of on-chip membranes • Develop and assemble hand-held chip reader and design • large-scale hard polymer chip production methods. • Test chip prototypes first using urine “spiked” with LAM, then urine • samples from TB patients (Kenya) and control samples.

  6. Detection Platform An open-flow impedance-sensing platform for LAM binding to a antibody functionalized carbon nanotube surface in the presence of a high-frequency AC electric field.

More Related