1 / 24

Comprehensive Germination Testing Methodologies and Results Analysis (2007-2009)

This document details the germination testing methodologies employed by Christoph Germeier and team from 2007 to 2009, including the processes for Fusarium counting and results analysis. It outlines specific germination methods used, like the ISTA methodology, the use of gibberellic acid for breaking dormancy, and variations in testing conditions. Key findings from the tests, including germination success rates and issues impacting germination like excess water, are discussed. This summary encapsulates the essential steps and outcomes of the germination tests conducted in various environments.

octavius-michael
Télécharger la présentation

Comprehensive Germination Testing Methodologies and Results Analysis (2007-2009)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Workpackage 02 Germination testing Christoph Germeier, Zofia Bulinska – Radomska, Izabela Borowska, Audrey Didier

  2. Germination methodology • Germination and Fusarium counting methodology • Germination test results 2008 • Germination methodology check test • Germination test results 2009 • germination test: how many accessions are being tested, when the tests have been started, how long do they all take?

  3. Germination methodology General outline of germination tests according to ISTA methodology: Between paper in climate chamber, Continuous light 20°C, 4 replications (recommended 8) a 50 seeds Breaking dormancy with gibberellic acid: GA3 (1000 mg/l) in phosphate buffer (1.7799 g Na2HPO4 x 2 H20 + 1.3799 g NaH2PO4 x H2O + 1 l aqua dest.) Count after 5 and 10 days Variants used in the project FRA 2007 / 2008: Round filter paper (diameter: 90mm) are moistened with gibberellin solution and placed in the petri plate then seeds are laid down onto the filter paper. A second moistened round filter paper is disposed on the top. POL 2007 / 2008: Filter paper envelopes (CHEMPUR, 65g/m2, 12,5x18,5 cm) are moistened with gibberellin solution. Seeds are placed into the envelope, incubated at 200C with continuous light.

  4. Germination methodology: Radzików

  5. Germination methodology: Radzików Mold Normal germ

  6. Germination methodology: Radzików Fusarium Normal germ

  7. DEU 2007 / 2008: Filter paper towels (e.g. Munktell C160, 15x60cm2) are pre-folded and moistened with gibberellin solution. Seeds are placed onto the fold, the towel then folded, rolled, stabilized in a plastic bag and incubated at 200C with continuous light. 2007: climate cabinet side of plastic bag open 2008: climate chamber top of plastic bag open

  8. Germination methodology: Quedlinburg Set up of germination tests in climate chamber (2008)

  9. Germination and Fusarium counting methodology: Quedlinburg • A) Abnormal germ and Molds

  10. Germination and Fusarium counting methodology: Quedlinburg • B) Fusarium more or less thick mycelium, reddish color, plants affected or not germinated

  11. Germination and Fusarium counting methodology: Quedlinburg • B) Fusarium more or less mycelium, reddish colour, plants not affected

  12. Germination and Fusarium counting methodology: Quedlinburg • B) Fusarium mainly reddish color, mycelium, symptoms not detectable

  13. Germination methodology check test (wild species)

  14. Germination methodology check test (marginally cultivated species)

  15. Germination methodology check test (cultivated oat) • Germination methodology check test

  16. Germination tests total summary

  17. Germination results species wise – wild species

  18. Germination results species wise

  19. Fusarium [%] results species wise – wild species

  20. Fusarium results [%] species wise – cultivated species

  21. Germination check results compared with germination test 2007/2008

  22. Germination check results compared with germination test 2007/2008

  23. Conclusion Main problem reducing germinability: Excess of water!

  24. Thanks for your attention

More Related