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Electroporation Innovation for Water Sterilization

Explore the pioneering application of electroporation in water sterilization, achieving a 100% kill rate of bacteria with safe E.coli K12. Follow the process from biosafety to manufacturing for clean and efficient water production.

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Electroporation Innovation for Water Sterilization

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  1. Dynamic Electroporation of Water Jacob Frye, Courtney Green, Christopher Robertson, and Scott Shields

  2. Introduction • Water is the most important resource. • chemicals(chlorine, iodine) are widely used for sterilization. • Development of alternative sterilization using electricity.

  3. Background • Electroporation – forcefully opening pores of bacteria. • Electrical team performed static electroporation tests. • Results more inclined towards repetition and low voltage.

  4. Background cont. • Use of E. coli k12 to minimize risk while properly modeling bacteria response to electroporation. • Group certification to handle high voltage sources.

  5. Escherichia coli K12

  6. Escherichia coli K12 • Safe and Harmless. • Similar Properties to Common or Lethal E. Coli • Optima Growth at 37 degrees Celsius • Kill rate exists at 42 degrees Celsius

  7. Biosafety Qualifications • To work in the biological lab, Scott and Jacob had to pass the biosafety lab examination and read the Biosafety lab manual • Strict guidelines for the type of clothing and to properly test E. coli K12 around other dangerous bacteria.

  8. Grow Process of E. coli K12 • Plate preparation • Growing process • Incubation period • Treatment and testing • Growing treated bacteria • Determine bacterial reduction

  9. Specific Phases of E. coli K12 • Lag Phase • Log/Exponential Phase • Stationary Phase • Death Phase

  10. Design Parameters • Use results from previous electrical team to develop mechanical flow system. • 100% kill rate of bacteria • 5 kV/cm³ to kill bacteria • Attain an 8 oz. cup of drinkable water in under 10 minutes

  11. Figure 1: Primary Design of Fluid Chamber

  12. Figure 2: Secondary Design (Implementation Pending Time Constraints)

  13. Figure 3: Peristaltic Pump used to generate flow

  14. Manufacturing • Prefabricated Acrylic Rectangle Tubing • Lathe turned connectors • 16 gauge silver sheet • 8 gauge silver rod lathe turned ends • Interference press fit connection • Silicon adhesive for water containment

  15. Figure 4: Fluid Chamber with silver plates connected to Peristaltic Pump

  16. Preliminary Testing • Determine the possible flow rates with the Peristaltic Pump. • Tested the chamber at maximum flow rate. • Tested the chamber at our design flow rate. • Video and Still Images to follow.

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