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  1. RNA and Protein Synthesis Trait Chapter 12 Section 3

  2. Why does the cell need to make proteins?

  3. REVIEW... DNA Replication Animation

  4. 1. RNA Ribonucleic acid Responsible for the movement of genetic information from the DNA in the nucleus to the site of protein synthesis in the cytosol

  5. A. Structure of RNA: • Single-stranded Nucleic acid made up of repeating units, like DNA • Sugar molecule is RIBOSE (not deoxyribose) • Thymine is replaced with URACIL (U)

  6. Compare & Contrast DNA RNA

  7. B. Forms of RNA • Messenger RNA (mRNA) a. STRUCTURE= RNA nucleotides in a single uncoiled chain b. FUNCTION= Carries genetic info from the nucleus to the ribosome

  8. Transfer RNA (tRNA) • a. STRUCTURE= RNA nucleotides in a single chain folded into a cloverleaf shape • b. FUNCTION= binds to specific amino acids and helps form polypeptide chains

  9. 3. Ribosomal RNA (rRNA) a. STRUCTURE = RNA nucleotides in a globular form b. FUNCTION = makes up the ribosomes where proteins are made

  10. 2. Protein Synthesis • The production of proteins • The amount and kind of proteins produced in a cell determine the structure and function of the cell • Protein’s carry out the genetic instructions encoded in an organism’s DNA

  11. Protein Structure • Proteins are POLYMERS • Made up of amino acids • There are 20 different amino acids that make up proteins

  12. Hydro- phobic Hydro- philic Acid Base

  13. a. Transcription

  14. T r a n s c r i p t i o n • What is it??? • making mRNA from DNA • NOTE: • Transcribe = to copy

  15. T r a n s c r i p t i o n Steps • RNA polymerase binds to DNA at the promoter and separates the DNA strands. • RNA polymerase then uses one strand of DNA as a template. Using the template as a guide, nucleotides are assembled into a strand of mRNA.

  16. Animation!

  17. DO NOW: Transcription • 1. Where does Transcription occur? • 2. What is produced at the end of transcription? • 3. What enzyme is used in transcription?

  18. b. T r a n s l a t i o n STEP 2 of PROTEIN SYNTHESIS

  19. T r a n s l a t i o n • The process TRANSLATING the message encoded in mRNA to assemble a protein. • Process begins when mRNA leaves the nucleus through pores in the nuclear membrane • The mRNA then migrates to a ribosome in the cytosol, the site of protein synthesis Animation

  20. THE GENETIC CODE • The genetic information necessary for making proteins is encoded in series of three mRNA nucleotides • Each combination of 3-mRNA nucleotides is called a CODON • Each codon codes for a specific amino acid

  21. Codons in mRNA tRNA carries the “Anticodon” . . . Allows tRNA to match up with mRNA codon

  22. If the mRNA sequence is A-A-U (codon) then the tRNA sequence is U-U-A (anticodon)

  23. T r a n s l a t i o n Steps • mRNA arrives at a ribosome • mRNA is pulled through the ribosome • Start codon signals tRNA to start arriving

  24. One tRNA matches up to the codons on mRNA to deposit an amino acid • Another tRNA comes in and adds another amino acid to the chain. • Process continues until stop codon is reached and enough amino acids have been linked to form a protein.

  25. T r a n s l a t i o n Product = Amino acids linked to form proteins

  26. Mutations • Mutations = errors • Mutations can occur as a “typo” in the sequence of nitrogen bases. • For example if a sequence should be A-C-G, a mutation could occur and it is now A-G-C . . . What affect could this have on the protein produced?

  27. PROTEIN SYNTHESIS ANIMATION Complete at stations... Transcribe & Translate a Gene Activity