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Leipzig, 23-25 April 2002 INRA, Avignon, France V. Chovelon, research scientist

Garlic and Health, P9 report. Leipzig, 23-25 April 2002 INRA, Avignon, France V. Chovelon, research scientist JP Leroux, research technician F. Mirrione, temporary agent (April 02). The objectives. Somatic embryogenesis plant regeneration and acclimatisation culture and evaluation

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Leipzig, 23-25 April 2002 INRA, Avignon, France V. Chovelon, research scientist

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  1. Garlic and Health, P9 report Leipzig, 23-25 April 2002 • INRA, Avignon, France • V. Chovelon, research scientist • JP Leroux, research technician • F. Mirrione, temporary agent (April 02)

  2. The objectives • Somatic embryogenesis • plant regeneration and acclimatisation • culture and evaluation • sanitary aspects • production of virus-free bulbs • meristem culture

  3. Somatic embryogenesis • milestones • Rouge Reunion, Messidrôme, Morasol: 200 plants from callus and 200 plants from cell suspension • in vitro culture, acclimatisation • field culture, evaluation • flow cytometry (P8), AFLP analyses (P1), • biochemistry (P5) • morphological and physiological notations (P8,P9) • deliverables • paper on regeneration of garlic plants issued from embryogenic callus (done) • paper on regeneration and acclimatisation of garlic plants issued from cell suspension (in progress)

  4. Rouge Reunion • plants from callus and cell suspensions • culture in tunnel • AFLP analyses (P1) • flow cytometry • phenotypic notations • harvest at maturity - field evaluation at Reunion Island (P8)

  5. In vitro culture Morasol plants from callus or cell suspension

  6. In vitro culture • Messidrôme plants issued from callus

  7. Acclimatisation and field culture • Morasol plants from callus and cell suspension

  8. Acclimatisation and field culture • Messidrôme plants issued from callus

  9. In vitro bulb production • factors for bulb induction and enlargement - Morasol, Messidrôme, Printanor: plants issued from cell suspensions - saccharose concentration: 40-60g/l - cold treatment: 5 weeks at 5°C - quality of light: additional far-red light - photoperiod: 12 or 16h - temperature: 22°C/night - 24°C/day

  10. Sanitary aspects • production of virus-free plants • 300 plants/variety • Messidrôme, Morasol • Printanor, Rouge Reunion • virological control • all the plants are potyvirus-free • some one contained latent viruses • Messidrôme: 100% GCLV, 34% MbFV • Morasol: 5.6% GCLV, 83% MbFV • Printanor: 100% MbFV • alliin analyses

  11. Sanitary aspects • virus elimination by meristem culture

  12. Workplan for 2002-03 • Embryogenesis, genetic characterisation • Rouge Reunion • field evaluation (P8), biochemistry (P5) • Messidrôme, Morasol from callus • field culture (P9), flow cytometry (P8), AFLP (P1) • Printanor from callus • in vitro culture, acclimatisation • Messidrôme, Morasol and Printanor from cell suspension • in vitro bulbs production • Sanitary aspects • production of virus-free bulbs (P9) • meristem culture (P9) acclimatisation, field culture

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