1. 120 110 100 54.8% 100% 54.8% 100.0% 90 80 70 60 50 60.9% 20.2% 40 30 p<.05 20 * % of Colonies per 12 High Powered Fields 10 p<.01 ** 0 + – – – DMSO/SCRB siRNA – + – – DMSO/PEA3 siRNA – – + – GSI/ SCRB siRNA – – – + GSI/ PEA3 siRNA A. C. DMSO/PEA3 siRNA DMSO/SCRB siRNA MDA-MB-231 XENOGRAFTS 3.0 Vehicle + Controli 2.5 Vehicle + PEA3i MRK-003 GSI + Controli 2.0 PEA3i + MRK-003 GSI Cross Sectional Area (cm2) 1.5 1.0 ** p=.031 0.5 * p=.016 0.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 GSI (MRK-003) SCRB siRNA GSI (MRK-003) PEA3 siRNA Weeks D. B. 1.2 1.0 0.8 Relative PEA3 Transcripts 0.6 0.4 0.2 0.0 Controli PEA3i