1 / 50

THE IMPORTANCE OF LABELLING YOUR S(TUFF)

THE IMPORTANCE OF LABELLING YOUR S(TUFF) In the Globe and Mail this weekend there was a little anecdote that went as follows: PhD student spends three years in tropical rainforest laboriously collecting lizard excrement for some sort of analysis.

pravat
Télécharger la présentation

THE IMPORTANCE OF LABELLING YOUR S(TUFF)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. THE IMPORTANCE OF LABELLING YOUR S(TUFF) In the Globe and Mail this weekend there was a little anecdote that went as follows: PhD student spends three years in tropical rainforest laboriously collecting lizard excrement for some sort of analysis. The PhD student stores the excrement in the lab. freezer in an unlabelled plastic bag. The PhD student returns to rainforest to collect more excrement (apparently this stuff is difficult to get!) The PhD student returns home. Finds out all his excrement has been thrown out in a lab clean-up. PhD student says his career and life are ruined. Let this tragedy be a warning to us all.

  2. THE RER/GOLGI SYSTEM

  3. The Nobel Prize in Physiology or Medicine 1974."for their discoveries concerning the structural and functional organization of the cell" George Palade LYSOSOMES/PEROXISOMES INTRACELLULAR PATHWAY OF SECRETORY PROTEIN TRAFFIC Christian de Duve

  4. Charles Leblond, McGill University Marian Neutra, Harvard Medical School ROLE OF THE GOLGI BODY

  5. Palade is credited with being the first one to describe the small particles that later came to be known as ribosomes.

  6. HOW DID PALADE AND CO-WORKERS ELUCIDATE THE PATHWAY OF SECRETORY PROTEIN TRAFFIN IN CELLS? HOW DID NEUTRA AND LEBLOND ELUCIDATE THE ROLE OF THE GOLGI BODIES IN PPROTEIN SECRETION?

  7. CHOOSE THE BEST CELLS TO WORK WITH! DIGESTIVE CELLS OF PANCREAS PALADE AND CO-WORKERS NEUTRA AND LEBLOND SMALL INTESTINE

  8. FIBROBLAST OSTEOCYTE

  9. (1) CHOOSE THE BEST CELLS TO WORK WITH! DIGESTIVE CELLS OF PANCREAS PALADE AND CO-WORKERS NEUTRA AND LEBLOND SMALL INTESTINE (2) USE THE BEST TECHNIQUES MICROAUTORADIOGRAPHY

  10. DUODENUM DUCT ZYMOGEN GRANULES BASEMENT LAMINA ACINAR CELLS ROUGH ENDOPLASMIC RETICULUM MITOCHONDRIA

  11. β-PARTICLES 3H HELIUM + β-PARTICLE

  12. USE THE AMINO ACID LEUCINE IN WHICH AN R-GROUP 1H-ATOM HAS BEEN REPLACED WITH AN 3H-ATOM

  13. SECRETORY VESICLES WITH PROTEIN MADE DURING THE EXPERIMENT SECRETORY VESICLES

  14. DECAY TRACK OF ONE β-PARTICLE

  15. www.ncbi.nlm.nih.gov/bookshelf/picrender.fcgi...

  16. PULSE-CHASE EXPERIMENT

  17. Charles Leblond, McGill University Marian Neutra, Harvard Medical School ROLE OF THE GOLGI BODY

  18. WANTED TO STUDY ROLE OF GOLGI BODY USED 3H-FUCOSE (A SUGAR) INSTEAD OF 3H- LEUCINE (AN AMINO ACID)

  19. GOBLET CELLS FROM EPITHELIUM OF SMALL INTESTINE

  20. GOBLET CELLS OF RAT INTESTINE

  21. THE MECHANISM OF POLYPETIDE IMPORT INTO THE RER

  22. Post-translational import PAGE 697

  23. CENTRAL VACUOLE MELANOSOMES Co-translational import INTEGRAL MEMBRANE PROTEINS P 687

  24. GUNTHER BLOBEL SIGNAL HYPOTHESIS

  25. HOW ARE THEINTEGRAL MEMBRANE PROTEINS INSERTED INTO THE RER MEMBRANE?

  26. p703

  27. p702 THE “STOP TRANSFER SEQUENCE” CAN SERVE AS THE SIGNAL SEQUENCE

  28. TRANSLOCON HOW THE POLYPEPTIDE CAN MOVE LATERALLY OUT OF THE TRANSLOCON

  29. THE ROLE OF CHAPERONES IN POLYPEPTIDE IMPORT INTO THE RER

  30. HEAT SHOCK PROTEINS IN FRUIT FLIES Hsp = HEAT SHOCK PROTEIN

  31. CHROMOSOMAL PUFFS POLYTENE CHROMOSOME ENDOMITOSIS MULTIPLE SISTER CHROMATIDS

  32. Hsp90 CHAPERONES (NOT BARRELS) CHAPERONINS (BARRELS)

  33. mG (AAAAA)n PORE PROTEIN OF TRANSLOCON Hsp70 ATP COO- NH3+ ADP +Pi NH3+ FOLDED PROTEIN

  34. Elizabeth Craig UNIVERSITY OF WISCONSIN

More Related