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Regulation of lipid storage in Saccharomyces cerevisiae

Regulation of lipid storage in Saccharomyces cerevisiae. Shaelie Harper McDonough-Stukey Lab. A Little History . How do cells “know” when they have made enough lipid? Previously, Cheryl Jacobs had isolated a mutant (114) we believe is defective at sensing lipid amounts

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Regulation of lipid storage in Saccharomyces cerevisiae

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  1. Regulation of lipid storage in Saccharomyces cerevisiae Shaelie Harper McDonough-Stukey Lab

  2. A Little History • How do cells “know” when they have made enough lipid? • Previously, Cheryl Jacobs had isolated a mutant (114) we believe is defective at sensing lipid amounts • My work this summer was to begin characterization of the mutant

  3. Growth Phenotype(clumpy vs. normal) • Within a tetrad, it can vary what strains are clumpy and which aren’t • If the ratio is 2:2 within a tetrad, this means there is only 1 mutation Clumpy Normal

  4. Gas Chromatography data • To the top is a picture of the GC at Hope College • To the left is an example of the data collected by the GC • Each Peak represents a different fatty acid, and its relative amount present in each cell

  5. Data Tables • By creating data tables, it is easier to make comparisons and conclusions for each individual strain

  6. Fatty acid Amount and Composition • These charts are compiled after analyzing the data from the GC and organizing it onto spreadsheets (shown on the previous slide), so we can compare different strains

  7. Result summary • Clumpy Growth is inherited in a single gene manner (2:2 inheritance) • Preliminary results indicate that elevated • fatty acid amount is correlated to growth • habit, and is a single gene mutation

  8. Conclusions • By examining inheritance patterns we are able to see • Clumpy growth habit is correlated to elevated FA amount • Elevated FA amount is a recessive single gene trait

  9. Credits • REACH • Dr. McDonough and Dr. Stukey • Rachel Van Kempen • Caitlin Rice

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