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D COOH

~12Kb. ATG. TGA. B. B. B. B. B. B. B. B. B. B. B. PGKneo. PGKneo. PGKneo. P. P. P. 12Kb. 4.5Kb. pA. pA. 8.5Kb. 4.5Kb. C. A. D COOH/ D COOH. D NH 2 / D NH 2. PM/PM.  / D COOH. AD1. +/PM. AD2.  / D NH 2. wt. wt. wt. P. P. P. P. P. P. P. Nkx2-1. P. P.

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D COOH

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  1. ~12Kb ATG TGA B B B B B B B B B B B PGKneo PGKneo PGKneo P P P 12Kb 4.5Kb pA pA 8.5Kb 4.5Kb C A DCOOH/DCOOH DNH2/DNH2 PM/PM /DCOOH AD1 +/PM AD2 /DNH2 wt wt wt P P P P P P P Nkx2-1 P P P P 1 372 DNH2 372 159 DCOOH 1 221 D 1 372 PM /DCOOH DCOOH /DNH2 wt DNH2 wt B pr Wt Wt Mutant Transfected cells Thyroid DNH2 pA E - + - + 1 2 3 4 phosphatase DCOOH native dephosphorylated PM PM/PM lung WT lung

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