'Transfected cells' diaporamas de présentation

What was the biological question addressed? What major claims to the authors make?

What was the biological question addressed? What major claims to the authors make? What are the major results that the authors obtained? Did the experiments presented justify the claims made? What major unanswered questions were raised by this work?

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Transfection

Transfection. Key words: Transient transfection, Stable transfection, transfection methods, vector, plasmid, origin of replication, reporter gene/ protein, cloning site, promoter and enhancer, signal peptide, polyadenylation signal.

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Fluorescence Correlation Spectroscopy (FCS)

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The Notch- ?-Secretase Pathway

The Notch- ?-Secretase Pathway. The Notch- ?-Secretase Pathway.

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? v ? 3 (FITC) and F-actin (TR)

Supplemental figure. A. Wt b 3-2 b 3-3 b 3-4 b 3-5 b 3-6 mock mock mock mock. B. NP-9 ? 3 -3. NP-9 ? 3 -4. NP-9 ? 3 -5. NP-9 ? 3 -6. ? v ? 3 (FITC) and F-actin (TR). Supplemental figure. NP-9 ? 3 clones: expression of ? 3 and cell morphology.

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EtBr ( ? g/ml)

GFP (%).

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Nonspecific siRNA Noxa siRNA

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10A. z

a. b. 10A. z p85 S83 ?. 10A. z p85 WT. 10A.vec. 10A. z. 10A.vec. 10A z. 10A z .p85 WT. 10A z .p85 S83 ?. E-cadherin. Vimentin. p85. p53. P-Akt. T-Akt. b -actin. 14-3-3 z. c. d. 10A.vec. 10A. z. 10A.vec. 10A. z. -. -. -. -. LY294002. 24hr. 48hr. 24hr. 48hr.

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D COOH

~12Kb. ATG. TGA. B. B. B. B. B. B. B. B. B. B. B. PGKneo. PGKneo. PGKneo. P. P. P. 12Kb. 4.5Kb. pA. pA. 8.5Kb. 4.5Kb. C. A. D COOH/ D COOH. D NH 2 / D NH 2. PM/PM. ? / D COOH. AD1. +/PM. AD2. ? / D NH 2. wt. wt. wt. P. P. P. P. P. P. P. Nkx2-1. P. P.

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