RNA-based Gene Regulation System: Riboswitch Functions & Mechanisms
Discover the roles of a metabolite-binding aptamer domain and an expression platform in gene regulation through riboswitch functions. Learn about direct metabolite binding to RNA, metabolite-dependent gene regulation, and RNA integrity modulation. Explore how ligand binding can influence mRNA cleavage, splicing, and stability. Uncover diverse riboswitch ligands and potential applications. Includes Affy GeneChip manufacturing details.
RNA-based Gene Regulation System: Riboswitch Functions & Mechanisms
E N D
Presentation Transcript
A metabolite-binding ‘aptamer’ domain and … … an ‘expression platform’ for gene regulation. Riboswitch Functions • Direct (protein-free) binding of metabolite to RNA • Metabolite-dependent regulation of genes These two activities are accomplished by two functionally separate domains on the RNA: X 1 2 3 4 U U U U U Courtesy: Ron Breaker Lab (Yale University)
X 5´ ORF A U G X GUACGG AG ORF A U G 5´ X ORF A A A A A A U G 5´ Riboswitch Mechanisms They also affect RNA integrity, and perhaps splicing and stability. Ligand binding leads to mRNA cleavage by a new natural ribozyme. X Ligand binding could control splicing in eukayotes. Possibly, ligand binding to the 3´ untranslated region could affect mRNA stability. Courtesy: Ron Breaker Lab (Yale University)
Riboswitch Ligands There are 8 confirmed riboswitches with unique metabolite ligands. Many more conserved RNA motifs are currently under investigation. Courtesy: Ron Breaker Lab (Yale University)
