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Enzyme kinetics

Enzyme kinetics. Cytochrome P450 bound to Its substrate camphor. L-serine + indole derivative produces L-tryptophan . Lock and key model. Proposed by Emil Fischer in 1890. Induced fit model. K +1 [E][S] = k -1 + k 2 [ES]. K M. If, Then it is independent of both

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Enzyme kinetics

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  1. Enzyme kinetics

  2. Cytochrome P450 bound to Its substrate camphor

  3. L-serine + indole derivative produces L-tryptophan

  4. Lock and key model • Proposed by Emil Fischer in 1890

  5. Induced fit model

  6. K+1[E][S] = k-1 + k2[ES]

  7. KM • If, • Then it is independent of both • Substrate concentration • Enzyme concentration

  8. X [S]

  9. X X X

  10. If = Then Thus V = Vmax/2

  11. X Thus Large KM weak binding, small KM strong binding Under these conditions KM tells us the enzyme-substrate affinity

  12. Significance of Vmax

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