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Analysis of protein fold changes in light and heavy backgrounds, with observed alterations in phosphorylation levels. Direct and inverted conditions compared showing diverse phosphosite modifications.
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Fold change: 1.11 [1.03 1.20] p TPSLPTPPTR K Background T Direct Light T Mixt T Heavy S [Observed] Fold change: -1.01 [-1.7 2.5] Fold change: 2.49 [2.05 3.19] TDHGAEIVYK p SPVV p SGDT p SPR K Direct Background Direct T Inverted Light T Mixt ASGQAFELIL p SPR T ASGQAFELIL p SPR K Heavy K Background Background S [Observed] S0 S1 S2 S3 S4 S5 S6 S7 S8 T T Light m/z [1 Da per step] Light p TPSLPTPPTR K T T Background Mixt Mixt T T T Inverted Heavy Light Heavy S [Observed] S [Observed] T Mixt T Heavy S [Observed] S0 S1 S2 S3 S4 S5 S6 S7 S8 TDHGAEIVYK p SPVV p SGDT p SPR K Inverted Background S0 S1 S2 S3 S4 S5 S6 S7 S8 S0 S1 S2 S3 S4 S5 S6 S7 S8 T Light T Mixt T Heavy S0 S1 S2 S3 S4 S5 S6 S7 S8 S [Observed] S0 S1 S2 S3 S4 S5 S6 S7 S8 Direct Inverted