'Fold change' diaporamas de présentation

Day 2 mAdb Analysis Tools

Course #412 Analyzing Microarray Data using the mAdb System July 15-16 , 2008 1:00 pm - 4:00pm madb-support@bimas.cit.nih.gov. Day 2 mAdb Analysis Tools. Use web site: http://mAdb-training.cit.nih.gov User Name on your card Password on the board. Esther Asaki, Yiwen He. Agenda.

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GeneChip analysis

GeneChip analysis. Microarrays Statistics Physics Biology. Andrew Harrison, UCL + Essex London Pain Consortium harry@biochem.ucl.ac.uk. Microarrays (mRNA expression). Microarrays are a massively-parallel Northern Blot.

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Differential Gene Expression Dennis Kostka, Christine Steinhoff

Differential Gene Expression Dennis Kostka, Christine Steinhoff. Slides adapted from Rainer Spang. Patients, Samples, Timepoints. Genes. Two cell/tissue /disease types: wild-type / mutant control / treated disease A / disease B responding / non responding etc. etc....

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Differentially expressed genes

Differentially expressed genes. 09/19/07. Identify differentially expressed genes. Fold Change. Based on the expression index, select genes with high fold change (e.g., R/G > 3) Advantage: Intuitive Larger fold change may indicate greater biological impact. Drawback

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MCB 317 Genetics and Genomics

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Differential gene expression detection, linear models, and Bayesian estimation with limma

Differential gene expression detection, linear models, and Bayesian estimation with limma. Richard Bourgon 16 June 2009 bourgon@ebi.ac.uk. Overview. Differential expression detection Fold change vs. t statistics Bayesian estimation and moderated t statistics

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Develop mathematical, statistical, and computational methods

Develop mathematical, statistical, and computational methods to analyse biologically or technologically novel experiments in order to understand disease-relevant regulatory and genetic interaction networks. What we do.

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Corporate presentation, BIO, Chicago 2013

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Amino acids 1

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New Testament Christianity

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Fig. 1 Supplement

Fig. 1 Supplement. 70.00. 60.00. 50.00. Fold change. 40.00. mir-10a. mir-130a. 30.00. 20.00. 10.00. 0.00. HELA. MCF7. 293A. JURKAT. NIH/3T3. HL60. COS7. Fig. 2 Supplement. 120. 100. 80. NIH/3T3 HyTK. NIH/3T3 TKpuro. Percentage. 60. NIH/3T3 TKzeo. NIH/3T3 Control. 40.

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Supplemental Table S1

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Supplemental figure 1

Supplemental figure 1. A. C. Plasma. B. Plasma. Serum. Serum. Gender -associated (50). Gender -associated (45). BMI dependent (7). BMI dependent (12). 2. 5. 48. 2. 10. 43.

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tnf- a (fold change)

Figure S1. D. A. IL-12 (pg/ml). il-12 (fold change). Cytokine (pg/ml). mRNA levels. B. E. IFN g (pg/ml). ifn- g (fold change). Donor 1. Donor 1. Donor 1. Donor 1. Donor 1. Donor 1. Cytokine (pg/ml). Donor 2. Donor 2. Donor 2. Donor 2. Donor 2. Donor 2. mRNA levels. C.

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E2F Probe

GSNO. GSNO. Cold Cyclin A. Cold Cyclin B. Cold CDC2. Cold PLK. mut PLK. 2.0. GSH. Cold E2F1. Anti-E2F1. Cold CDC2. GSNO. Cold CDC6. mut E2F1. PBS. GSNO. GSH. PBS. 1.8. 1.6. 1.6. 1.4. 1.4. Fold change. 1.2. 1.2. 1.0. Fold change. 1.0. 0.8. 0.8. 0.6. 0.6. A. B.

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MTT Assay (Fold change)

MTT Assay (Fold change). MTT Assay (Fold change). - 12,5 25 50 100. GW 4064 µM. - 0,3 3 6,5 10. a. b. MCF-7 TR2. MCF-7 TR2. CDCA µM. c. HER2. β -Actin.

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H+E p53

a. H+E p53. Cre+Apc fl/fl P53 +/+. b. QRT-PCR expression of P53. 2.5. *. 2. 1.5. Fold Change. 1. *. *. 0.5. WT ApcFlox Double P53null. 0.

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Fold-change

Ereg. Tnc. 200. 60. p<0.001. 150. p<0.001. 40. p<0.002. 100. p<0.002. 20. 50. 0. 0. Oil. 1. 3. Oil. 1. 3. (normalized to BALB oil). (normalized to BALB oil). Fold-change. Fold-change. Ccl17. Pparg. BALB. BALB. BALB. BALB. BALB. BALB. BALB Lpsd. BALB Lpsd.

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Fold-change

Col18a1. Cfi. 60. 30. BALB. BALB. BALB. BALB. BALB. BALB. BALB. BALB. 50. 25. BALB Lpsd. BALB Lpsd. BALB Lpsd. BALB Lpsd. BALB Lpsd. BALB Lpsd. BALB Lpsd. BALB Lpsd. p<0.001. p<0.001. 40. 20. (normalized to BALB. (normalized to BALB. (normalized to BALB.

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Supplemental Figure 4

A. Panc5.04. A10.7. A38.44. ***. **. ***. ***. ***. **. **. **. B. C. Panc5.04. A38.44. ADM NDRG-1. ***. **. **. Vehicle (10%FBS) Vehicle (1%FBS) SB202190 (10%FBS) SB202190 (1%FBS). Vehicle (10%FBS) Vehicle (1%FBS) SB202190 (10%FBS) SB202190 (1%FBS). Vehicle (10%FBS)

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