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Comparative Pro-inflammatory Cytokine Responses in Human PBMCs to Reference Strains

This figure presents an analysis of the pro-inflammatory transcriptional responses of human PBMCs from two donors, illustrating cytokine levels (IL-12, IFN-γ, and TNF-α) 4 hours post-stimulation with reference and three strains of Propionibacterium freudenreichii (MOI of 10). It shows fold changes in mRNA levels and corresponding cytokine responses measured in pg/ml. The methodology mirrors that previously detailed in Figure 1, emphasizing key immune responses to specific bacterial strains at the 24-hour mark.

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Comparative Pro-inflammatory Cytokine Responses in Human PBMCs to Reference Strains

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  1. Figure S1 D A IL-12 (pg/ml) il-12 (fold change) Cytokine (pg/ml) mRNA levels B E IFNg(pg/ml) ifn-g (fold change) Donor 1 Donor 1 Donor 1 Donor 1 Donor 1 Donor 1 Cytokine (pg/ml) Donor 2 Donor 2 Donor 2 Donor 2 Donor 2 Donor 2 mRNA levels C F tnf-a (fold change) TNFa (pg/ml) Cytokine (pg/ml) mRNA levels

  2. Figure S1. Comparative pro-inflammatory transcriptional responses of human PBMCs (2 donors) for reference strains for il-12, ifn-γ and tnf-α (A, B, C) 4 h after stimulation with references and 3 P. freudenreichii strains (MOI of 10) and the corresponding TNFα, IL-12 and IFNγ cytokine responses (D, E, F) at 24 h. Methodology is similar as described for Fig. 1.

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