Download
1 / 1
10 likes | 127 Vues
This study investigates the osmolarity control mechanisms in hyperekplexic α1R271Q-GlyR and wild-type α1GlyR using different osmotic agents. We analyze the effects of 600 mM sucrose and 1100 mM glucose on glycine responses under controlled conditions. Experiments include varying concentrations of glycine (1 mM, 10 µM, and 300 mM) and assess the impact on receptor activity measured at 50 pA over specified durations. Our results provide insights into the osmotic influences on receptor function in a neuromuscular context.
E N D
600 mM sucrose (40s) 10 µM glycine 1100 mM glucose (2 s) 1 mM glycine (2 s) 300 mM glycine (1 s) 50 pA 50 pA 25 s 25 s Additional file 1: Controls of osmolarity at the hyperekplexic α1R271Q-GlyR with 1100 mM glucose, (n=4) Controls of osmolarity at the α1GlyR (WT) with 600 mM sucrose, (n=6) 600 mM sucrose (10s) 10 µM glycine
