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I cant talk, I have laryngitis

I cant talk, I have laryngitis . Pay Attention so I can “explain” the lab. 1 st : girls tie your hair back and get on your closed toed shoes

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I cant talk, I have laryngitis

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  1. I cant talk, I have laryngitis

  2. Pay Attention so I can “explain” the lab • 1st: girls tie your hair back and get on your closed toed shoes • 2nd: you always start and end a microbiology lab by spraying all surfaces down with 70% Ethanol (EtOH) and wiping with paper towels so somebody go do that

  3. Everybody get a glass slide and using a sharpie draw a circle in the center of the slide • IMPORTANT flip the slide over so you are using the side that does not have ink. This is important or when you decolorize later it will bleed

  4. Get a metal loop, flame it, let it cool, dip it in water and add 1 small loop full of water to the center of the circle you drew • Flame your loop again, choose a colony to pick up off of one of the petri dishes, and once you have it swirl it in the drop of water on your slide to dislodge your colony • Let it air dry

  5. Step 1: heat Fix • Using a clip to hold your slide pass the slide QUICKLY 3 times through the flame • DO NOT SET YOURSELF ON FIRE!!!!!

  6. Flood the slide with crystal violet solution for up to one minute. Wash off briefly with tap water (not over 5 seconds). Drain. • Flood slide with Gram's Iodine solution, and allow to act (as a mordant) for about one minute. Wash off with tap water. Drain.

  7. Remove excess water from slide and blot, so that alcohol used for decolorization is not diluted. Flood slide with 95% alcohol for 10 seconds and wash off with tap water. (Smears that are excessively thick may require longer decolorization. This is the most sensitive and variable step of the procedure, and requires experience to know just how much to decolorize). Drain the slide.

  8. Flood slide with safranin solution and allow to counterstain for 30 seconds. Wash off with tap water. Drain and blot dry with bibulous paper. Do not rub.All slides of bacteria must be examined under the oil immersion lens.

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