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High-Performance Liquid Chromatography of Acetylsalicylic Acid Using ELSD

This application note discusses the method for analyzing Acetylsalicylic acid using High-Performance Liquid Chromatography (HPLC) coupled with an Evaporative Light Scattering Detector (ELSD). The analysis utilizes a Phenomenex Kinetex C18 column at an ambient temperature with a mobile phase of 50:50 Acetonitrile and water containing 0.2% TFA. The calibrated setup guarantees high linearity and sensitivity, even at low concentrations that are undetectable by the ELSD. This note includes chromatograms and is part of Application Note QAN-0069 from Quant Technologies.

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High-Performance Liquid Chromatography of Acetylsalicylic Acid Using ELSD

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  1. PARAMETERS NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 50:50 ACN:Water with 0.2% TFA APPLICATION NOTES FLOW RATE: 1.00 mL/min COLUMN: Phenomenex Kinetex C18, 150 x 4.6, 2.6 u COLUMN TEMP: Ambient INJECTION VOLUME :1 µL ANALYTE: Acetylsalicyclic acid ™ QUANT TECHNOLOGIES, LLC 1463 94TH Lane NE Blaine, MN, USA 55449 763-398-0508 www.Quant-NQAD.com THE NQAD IS HIGHLY LINEAR, EVEN AT LOW CONCENTRATIONS WHICH ARE UNDETECTABLE BY THE ELSD Application note # QAN-0069 CHROMATOGRAMS Note: This application was run at Quant Technologies on a single ELSD and may not be representative of all ELSD’s.

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