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Discover how to achieve lower concentrations using the NQAD detector in chromatography applications. This note features chromatograms, parameters, and specific gas and mobile phase details for optimal results.
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4.57 APPLICATION NOTES 5.80 45.0 0.00 1.00 2.00 2.5 ™ QUANT TECHNOLOGIES, LLC 1463 94TH Lane NE Blaine, MN, USA 55449 763-398-0508 www.Quant-NQAD.com 4.20 0.00 1.00 2.00 2.50 SEE LOWER CONCENTRATIONS WITH THE NQAD DETECTOR Application note # QAN-0086 CHROMATOGRAMS NQAD S/N 222.3 PARAMETERS NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 57:43 ACN:Water ELSD S/N 8.2 FLOW RATE: 1.25 mL/min COLUMN: Thermo Beta Basic C18, 150 x 4.6 mm, 5u COLUMN TEMP: Ambient INJECTION VOLUME :1 µL CONCENTRATION: 32 ng on column ANALYTE: SDS Note: This application was run at Quant Technologies on a single ELSD and may not be representative of all ELSD’s.