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Laboratory of Bioenergetics

Laboratory of Bioenergetics. main laboratory : 3.2.11, 3.2.11a climatic chamber : 3.2.11 animal rooms: 3.0.xx administrator: Paweł Koteja Evolutionary Physiology Research Team. Respirometric system Sable TR3. Main location: 3.2.11. oxygen analyzer - „Oxzilla”: - 2-channel

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Laboratory of Bioenergetics

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  1. Laboratory of Bioenergetics main laboratory : 3.2.11, 3.2.11a climatic chamber : 3.2.11 animal rooms: 3.0.xx administrator: Paweł Koteja Evolutionary Physiology Research Team

  2. Respirometric system Sable TR3 Main location: 3.2.11 • oxygen analyzer - „Oxzilla”: • - 2-channel • - 0-100%, res. 0.001%; • CO2 analyzer - CA2A • - 0-10%, res. 0.0001%; • continuous measurement on 1 or 2 ch. • sequential on 8-16 ch. • open flow-through orsemi-closed system; • from microorganism cultures (as Microoxymax) to large animals; • a small climatic chamber; • mobile

  3. Respirometric system Sable TR3 Main location: 3.2.11 Example of an open-flow setup: measurement of the maximum exercise-induced rate of aerobic metabolism

  4. Respirometric system Sable TR3 Main location: 3.2.11 Example of an open-flow setup: measurement of the maximum exercise-induced rate of aerobic metabolism

  5. More devices for respirometric measurements • O2 analyzer Applied Electrochemistry (AMETEK) S3/AII: • 2-channel, 0-100%, res. 0.001%, very fast, stable, lab. use only • 2 x O2 Sable analyzer FC-10A: • 1-channel: 0-100%, res. 0.001%, slow, not stable, mobile, 12V • CO2 analyzer Sable CA10A: • 1-channel, 0-10%, res. 0.0001%, fast, stable, mobile, 12V • CO2 and H2O analyzer LiCor LI-840A: • Low cost – "two in one", mobile, parameters similar to CA10A • Several pumps, mass flow controllers, channel multiplexers, air driers, etc.

  6. Motorized treadmillfor respirometric measurements • mouse-sized animals; • speed up to 6 km/h; • computer controlled speed regulation; • manual angle control • waterproof

  7. Activity detectors – passive infrared Custom made: 3M – Mouse Motion Monitor • 64 sensors; • expandable; • elastic system of data acquisition • time resolution 0.01 sec. • mobile – animal rooms, climatic chambers

  8. Activity detectors - mechanical Sable MAD- 1 ("seismographs") • 8 sensors; • elastic system of data acquisition • time resolution 0.01 sec. • mobile – animal rooms, climatic chambers

  9. Video system for behavioral analyses Noldus Ethovision • video cameras, multichannel recorders • analog-digital converter ("frame grabber") • video analysis system • main result – path of animal movement • detection of a few individuals in one arena • detection in several arenas (with one individual in each); • several characteristics of movement: speed, time in particular zones, "meandering"; • automated trials for standard behavioral tests: • open field • Morris water maze • multi-arm labyrinths

  10. Automatic extraction apparatusBüchi B-811 • 4-positions • temperature regulation up to +150C • extraction in neutral gas • automated: • phases of the process (extraction, rinsing, drying) • various types of extraction • programmable sequences

  11. Automatic extraction apparatusBüchi B-811 Types of process

  12. Hydrolysis apparatus Büchi B-811 • 4 positions • vials compatible with the extraction apparatus

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