1 / 12

Mutagenic Properties of Agricultural and Household Pesticides

Mutagenic Properties of Agricultural and Household Pesticides. DAVID FARRIS, KIMBERLY CHAMPINE, DAVID RICKS, JENNIFER SEABAUGH, and JIM CHAMPINE. Department of Biology, Southeast Missouri State University, Cape Girardeau, MO. Pesticide Usage in Southeast Missouri.

richardcrawford
Télécharger la présentation

Mutagenic Properties of Agricultural and Household Pesticides

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mutagenic Properties of Agricultural and Household Pesticides DAVID FARRIS, KIMBERLY CHAMPINE, DAVID RICKS, JENNIFER SEABAUGH, and JIM CHAMPINE Department of Biology, Southeast Missouri State University, Cape Girardeau, MO

  2. Pesticide Usage in Southeast Missouri • Over 150 pesticides are used in Scott and Dunklin Counties • Shared aquifer represents the drinking water supply • Anecdotal reports

  3. Inquiry • Do pesticides represent a health threat? • Test pesticides and ground water samples using the Salmonella Histidine-reversion assay (Ames test). • Null hypothesis – these materials do not increase the mutation rate of the indicator organism.

  4. His- strains of Salmonella typhimurium Exposure to the suspected mutagen Reversion to His+ on minimal medium Reversion rate = forward rate The Ames Test

  5. Procedure 0.1 ml Culture 0.1 ml Compound Top Agar 24 hours Glucose minimal medium, trace histidine 48 hours Plate Incorporation Test

  6. Tester Strain Genotypes

  7. Compounds Tested

  8. Results

  9. Conclusions • Round-up induces frameshift mutations • Bug-stop induces both frameshift and point mutations • Hot-shot does not induce frameshift mutations (data not shown)

  10. Discussion • Be careful handling pesticides! • Environmental Mutagen Information Center Index • Correlation between mutagenicity and carcinogenicity

  11. Future Research • Agricultural pesticides and ground water samples • “Activation” by S9 Liver extract • Synergistic effects

  12. Acknowledgements • Kimberly Champine and David Ricks • Maija Bluma and Krista LeGrand • John Kraemer • Southeast Missouri State Grants and Funding Committee

More Related