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Characterization of Signal Peptide and Transmembrane Domain in Zebrafish Gene Structure

This study explores the genomic structure of a zebrafish gene located on chromosome 15, focusing on its signal peptide, transmembrane domains, and the associated protein sequences. The analysis highlights consensus sequences within the V-type immunoglobulin domain and the presence of an intracytoplasmic C-terminal tail. We present detailed sequence data, including fundamental elements of gene expression and regulation, which may elucidate the functional characteristics of this gene in zebrafish.

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Characterization of Signal Peptide and Transmembrane Domain in Zebrafish Gene Structure

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  1. A Signal Peptide S1 S- N1,2 S2 DS* N1,2 Hsb2 Rnb2 zb2B 74 IG V-type consensus: x x ^ x ^ x C ^ x W G N1 N1,2 DS* Hsb2 Rnb2 148 zb2B x x D x & x Y x C ^ x ^ x x ^ x * ^ ^ Transmembrane GSCS Hsb2 Rnb2 zb2B 223 x ^ x ^ C zb2 genomic structure zebrafish chromosome 15, 27.3Mb, reverse strand 3’ 5’ 1 2 3 zb2A 1 2 3 4 5 6 zb2B 2 3 5 1 4 zb2C 1 2 3 4 5 zb2D 2.65kb D y y y y y y y y -s -s -s -s IG (V-type) s- s s- s s- s s- s y y y y NH3 NH3 NH3 NH3 CO2 A B C D 15aa 45aa 38aa CO2 CO2 CO2 Transmembrane Intracytoplasmic C-terminal tail zb2B zb2C zb2D B

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