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Impedance Spectroscopy for Toxicity Testing Martin Dominguez Cronus Technologies Ltd.

Impedance Spectroscopy for Toxicity Testing Martin Dominguez Cronus Technologies Ltd. Barrier Forming Tissue. Epithelial and endothelial cell layers form selectively permeable barriers. The Concept. Growing cell layers on permeable membranes. The Concept.

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Impedance Spectroscopy for Toxicity Testing Martin Dominguez Cronus Technologies Ltd.

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  1. Impedance Spectroscopy for Toxicity Testing Martin Dominguez Cronus Technologies Ltd.

  2. Barrier Forming Tissue Epithelial and endothelial cell layers form selectively permeable barriers

  3. The Concept Growing cell layers on permeable membranes

  4. The Concept Measuring the electrical impedance of epithelial and endothelial cell layers under physiological conditions

  5. Understanding Impedance Spectra Contributions stemming from CPE and RMED

  6. Understanding Impedance Spectra The formation of a plateau at mid-range frequencies is attributable to the cell layer’s resistance TER and capacitance CCL

  7. Understanding Impedance Spectra An increase in the resistance TER shifts the plateau upwards

  8. Understanding Impedance Spectra An increase in the capacitance CCL narrows the plateau

  9. Time-Lapse Impedance Spectroscopy

  10. The Setup

  11. The Setup

  12. cellZscope Automated cell monitoring device

  13. Monitoring of MDCK-II Cells Formation of a differentiated cell monolayer with established cell-cell junctions

  14. cellZscope vs. „Chopstick“ Electrodes Comparing TER values measured on MDCK-II cell layers

  15. Long Term Monitoring of Caco-2 Cells Comparing growth dynamics of different cell lines

  16. EDTA Treatment of MDCK-II Cells Effects on the tight junction network

  17. MBCD Treatment of MDCK-II Cells Dose dependent response in TER and Ccl to cholesterol depletion

  18. Testing the Toxicity of Nanoparticles Exposure of MDCK cells to different types of particles + Particles + Triton X100 180 160 Control 140 Control 120 Carbon Black* Carbon Black* 100 TER (%) ZrO2* 80 ZrO2* 60 ZnO - NP* ZnO - NP* 40 * 10 µg/cm² 20 0 0 5 10 15 20 25 Time (h) Data courtesy of A. Kroll, J. Schnekenburger, University of Münster, Germany

  19. Testing the Toxicity of Nanoparticles Exposure of NRK52E cells to ZnO + ZnO + Triton X100 120 100 Control Control 80 ZnO* ZnO* ZnO* TER (%) 60 ZnO NP* ZnO NP* ZnO NP* 40 * 10 µg/cm² 20 0 0 5 10 15 20 25 Time (h) Data courtesy of A. Kroll, J. Schnekenburger, University of Münster, Germany

  20. cellZscope Automated cell monitoring device

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