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Discover the varying sensitivity to manganese deficiency in winter barley genotypes and the importance of manganese efficiency for optimal growth. Learn about chlorophyll fluorescence as a diagnostic tool and the implications for field conditions. Gain insights from field trials and explore the challenges in interpreting data.
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Sortsforskelle i følsomhed overfor manganmangel Plantekongres 2005. C.A Hebbern
Mn efficiency: An ability to grow well on Mn deficient soil • Winter barley genotypes Vanessa and Antonia identified as Mn efficient and Mn inefficient. • In hydroponics, the genotypes did not differ in Mn concentration in shoot or root. • Vanessa had a higher uptake rate of Mn than Antonia; maybe important in field conditions.
Chlorophyll fluorescence • Reveals clear differences between Vanessa and Antonia
Vanessa and Antonia: hydroponics Control and Mn deficient plants clearly different with fluorescence. Genotypes also different to each other.
Important differences • Mn efficient Vanessa dissipates more light energy as heat • Mn inefficient Antonia is more chlorotic • Antonia may therefore be more at risk of oxidative stress, leading to reduced yield
Mn efficiency: can we be certain? • Field trials over four years have identified some consistent differences • Antonia is Mn inefficient • Vanessa and Carola are Mn efficient • These results confirmed at KVL • But field trial data can be difficult to interpret...
Field trial requirements • Control plots and Mn deficient plots must be clearly different • Soil must be Mn deficient • Control plots and Mn deficient plots must be on directly comparable soil (close neighbours!)
Field trials for Mn efficiency • We calculate Mn efficiency as (Mn- / Mn+) x 100, Where the Mn- and Mn+ plots are found opposite each other in the field trial (comparable soil). 100% efficiency: no difference in yield on Mn deficient soil after spraying
Field trials for Mn efficiency • Trials have so far used the following design:
Improving paired plot designs Zinc sprayed and unsprayed plots of barley, sown side by side. 1998, Horsham, Australia.
Conclusions • Identification of Mn inefficiency in Antonia • And probable Mn efficiency in Vanessa and Carola • Further study needed on the differences between Carola, Vanessa and Antonia • We must improve field trials for Mn efficiency.