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Enhancing CDOM Absorption Measurement: Protocols for Accurate Spectral Analysis

This guide discusses the importance of using a blank material in CDOM absorption measurements, particularly its advantages over traditional spectrophotometers. It reviews the necessary steps for proper calibration of sensors, the significance of maintaining clean water samples free of bubbles, and the effects of temperature and salinity on measurements. A suggested protocol outlines the measurement of absorbance and concentration of both blank water and CDOM samples, along with corrections for temperature and salinity, ensuring greater accuracy and reliability in data analysis.

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Enhancing CDOM Absorption Measurement: Protocols for Accurate Spectral Analysis

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  1. CDOM Absorption: ac9Emmanuel Boss

  2. ac9 10/25 cm Sensitivity better than spectrophotometers. Why? Reference (blank) material? Why do we need a blank ?

  3. Twardowski et al, 1997 (similar for c):

  4. How do we blank? • Important points: • Clean water w/o bubbles. • Calibrate sensors the same way they are deployed. • Take temperature reading (see soon).

  5. (1997) What should we use for reference temperature?

  6. How good is the assumption of a linear drift? • Suggested protocol: • Measure a and c of blank water. • Correct blank for temperature effects (relative to factory). • Measure a and c of CDOM • Correct for blank. • Correct for effect of temperature and salinity (relative to factory). How do we expect the a and c spectra of CDOM to look after all the corrections?

  7. Provides great check on calibration !

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