1 / 15

ACADEMIA SINICA IBMS

ACADEMIA SINICA IBMS. 嚴仲陽 美國德州貝勒醫學院 博士. Specialty: Molecular & Cell Biology Hematopoiesis Apoptosis Regulation. Method Yeast Two-hybrid. Material. Yeast strain ： L40 ( -his 、 -trp 、 -leu 、 -ade ) Plasmid ： pBTM116 ＆ pACT2 Competent Cell ： HB101 Yeast DNA purification kit

rusty
Télécharger la présentation

ACADEMIA SINICA IBMS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ACADEMIA SINICAIBMS 嚴仲陽 美國德州貝勒醫學院 博士 Specialty: Molecular & Cell BiologyHematopoiesisApoptosis Regulation

  2. MethodYeast Two-hybrid

  3. Material • Yeast strain：L40 (-his、-trp、-leu、-ade) • Plasmid：pBTM116 ＆ pACT2 • Competent Cell：HB101 • Yeast DNA purification kit • E coli. DNA purification kit

  4. Result • No Result ……

  5. Normal Transcription

  6. Yeast Two-hybrid Transcription

  7. Plasmid construction

  8. Bait ＆ Prey

  9. Bait－pBTM116

  10. Prey－pACT2

  11. Yeast Two-Hybrid

  12. Transformation ＆ Assay-Trp, -Leu, -His

  13. β-galactosidase assay

  14. gs3955 ＆ library • Yeast Transformation • Pick colonies • Selection three times • β-galactosidase assay • Yeast DNA purification • Transform to HB101 • Bgl Ⅱ check • Retransform to yeast • Sequencing

  15. My summer vacation • Experiment • Travel • Camp

More Related