1 / 27

Introduction to immunohaematology

2. Introduction to immunohaematology. BY Dr:Maha AL-Sedik. Objectives: 1- Rh blood group system. 2- Antigen and antibody of each blood group type. 3- Detection of the Rh blood group. 4- Importance of Rh blood group system. 5- Antihuman globulin ( Coombs , ) test.

rusty
Télécharger la présentation

Introduction to immunohaematology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 2 Introduction to immunohaematology BY Dr:Maha AL-Sedik

  2. Objectives: 1- Rh blood group system. 2- Antigen and antibody of each blood group type. 3- Detection of the Rhblood group. 4- Importance of Rh blood group system. 5- Antihuman globulin ( Coombs, ) test.

  3. Rh blood group system: As some red blood cells have an additional antigen (known as the RhD antigen), there is a further type of classification. If the red blood cells contain this antigen then they are classed as RhD +ve, and if they don't, are classed as RhD -ve. Anti Rh gene is of IgG type.

  4. This means that there are eight main blood types, these are: • A positive (A+) • A negative (A-) • B positive (B+) • B negative (B-) • AB positive (AB+) • AB negative (AB-) • O positive (O+) • O negative (O-) • There are 2 major subgroups of group A : A1 : 80% & A2 :20%

  5. Importance of Rh blood grouping: • Haemolytic transfusion reaction in case of Rh mismatched blood transfusion. • Acute graft rejection in case of Rh incompatible solid organ transplantation. • Haemolysis of donor red cells following Rh incompatible bone marrow transplantation. • Rh antibody can cause haemolytic disease of newborn. • Medicolegal importance.

  6. Antihuman globulin (coombs' test) Detection of non-agglutinating Ab , especially IgG or complement components (C3d) affixed to RBCs in vivo or in vitro.

  7. Direct antiglobulin test (DAT): detection of sensitization of RBC s (coated with Abs and/or complement components) in vivo. • Indirect antiglobulin test (IDAT): detection of sensitization of RBCs in vitro.

  8. Indirect Coombs test (Indirect Antiglobulin test): to detect presence of IgG or complment in patients serum. Indications: • To check whether an Rh-negative women (married to Rh-positive husband) has developed Anti Rh-antibodies. • Pre-transfusion of Rh positive blood to Rh negative recipient.

  9. Principle:

  10. Procedure:

  11. Result:

  12. Direct Coombs test (direct Antiglobulin test): The direct antiglobulin test (DAT) detects sensitized red cells with IgG and/or complement in vivo. Indications: • Hemolytic transfusion reaction. • Hemolytic disease of the newborn (also known as HDN or erythroblastosis fetalis).

  13. Principle:

  14. Procedure:

More Related