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MRC-5

Supplementary Fig. 2. MRC-5. MCF-7. (A). (B). (C). Mock P50. Mock P25 P50 P75 P100. Mock. p53. LC3-I. LC3-II. LC3-I. LC3-II. b -act. b -act. Prima-1.

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MRC-5

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  1. Supplementary Fig. 2 MRC-5 MCF-7 (A) (B) (C) Mock P50 Mock P25 P50 P75 P100 Mock p53 LC3-I LC3-II LC3-I LC3-II b-act b-act Prima-1 Induction of autophagy in MCF7 and MRC5 cells. A) MCF7 cells were mock-treated or treated with 100 µM PRIMA-1 for 24 h and fixed for immunostaining with an anti-LC3 antibody. Bar 10mm.B) Western blots showing LC3-I and LC3-II bands in PRIMA-1-treated cell lysates. C) Non-tumor lung fetal MRC-5 cells were mock-treated or treated with 25-100 µM PRIMA-1 for 24 h and processed as described in Materials and Methods. Western blots showing p53, LC3-I and LC3-II bands are presented.

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