Lecture 16 Chapter 9 Promoters Neal Stewart
Objectives • What heterologous promoters are used to make transgenic plants, and why? • Why are constitutive promoters so popular? • What additional vector considerations should be considered given the nature of strong constitutive promoters?
Types of cassettes Figure 9.1
Figure 9.5 Promoter and enhancer trapping
Figure 9.6 Strong heterologous promoters might have “bleed-over” effects
What would be the properties of an ideal promoter? • Broad range of expression in a number of diverse species • Of plant origin • Maybe constitutive • High expression • Tissue specificity • Developmental specificity • Inducibility • Compact—short sequence