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Microtubule motors

Microtubule motors. Text and image sources are included using the notes function of this file. Intracellular transport vesicles. VSVG-GFP and Microtubules. Fast Axonal Transport. Loligo. in vivo in vitro. Freeze!. Transport vesicle. kinesin. microtubule. 50 nm.

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Microtubule motors

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  1. Microtubulemotors Text and image sources are included using the notes function of this file

  2. Intracellulartransport vesicles VSVG-GFP andMicrotubules

  3. Fast Axonal Transport Loligo in vivo in vitro

  4. Freeze! Transport vesicle kinesin microtubule 50 nm

  5. Kinesin develops about 6 picoNewtons (pN) of force Biophysical analysis 8nm steps correspond to the spacing of tubulin dimers

  6. Conventional KinesinTwo heads are better than one! ATP

  7. Kinesin model

  8. Fluorescent microtubules gliding over a kinesin-coated surface In vitro motility Kinesin

  9. Biotech saddles the beast A “rectifying” shape can direct traffic

  10. Kinesins share a conserved motor domain, placed in different evolutionary contexts 100 nm

  11. Turning tail 400kDa Conventional Kinesin Ionic strength (in vitro) Post-translational modification? Folding modulates interactions with microtubules and cargo

  12. Kinesin in vitro motility assays seed Using polarity marked microtubules - + Conventional kinesin is a plus-end directed motor (minus-end of track leads) Ncd is a kinesin that side steps in a weakly minus-end directed motion to rotate the track

  13. Stepping out

  14. Kinesin family tree

  15. MAPs can block motor activity Kinesin-driven anterograde movement of mitochondria is blocked by ectopic expression of MAPs like Tau

  16. Cytoplasmic dynein

  17. Adapting to a membrane

  18. Cytoplasmic dynein and b-COP

  19. Dynein inhibition causes outward dispersal of lysosomes

  20. Dynein family tree Spotty, perinuclear localization Flagellar dyneins Golgi localization

  21. Chlamydomonas

  22. Flagellar structure

  23. The flagellar dynein motor is (a) complex

  24. Microtubule sliding powers flagellar beating

  25. Sliding disintegration

  26. Dynein Mechanochemical Cycle

  27. Axonemal abnormalities Normal Kartagener’s syndrome

  28. Keeping the beat!

  29. Regulation

  30. Building the flagellum

  31. Microtubule commuters kinesin dynein

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