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Applications of the Immune Response

Applications of the Immune Response

Applications of the Immune Response. Hugh B. Fackrell Filename JWR12bv7.ppt. Content Outline. Radioimmunoassay (RIA) Enzyme Linked Immunosorbent Assay (ELISA) Western Blots Immunofluorescence Immunoelectron Microscopy. Immunoadsorbent Assays. E nzyme L inked I mmuno S orbent As say

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366 views • 20 slides
Simple ELISA protocol

Simple ELISA protocol

Simple ELISA protocol. 1. Coat antigen onto microplate. 2. Allow protein adsorption and block unoccupied sites with neutral protein. 3. Add antibody solution into each well.

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471 views • 13 slides
2-DE

2-DE

https://www.creative-proteomics.com/services/2d-electrophoresis.htm

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2D Electrophoresis

2D Electrophoresis

Creative Proteomics offers an integrated solution to the identification of low abundance proteins in complex biological sample through 2D electrophoresis. This technique sorts proteins according to two independent properties in two discrete steps. Each spot on the resulting two-dimensional array corresponds to a single protein species in the sample. Thus thousands of different proteins can be separated, and the information is obtained including the protein pI, the apparent molecular weight, and the amount of each protein.

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1471 2091 14 28 s1

1471 2091 14 28 s1

Additional file 1. Visualization of native and His-tagged YedY on 2D gel electrophoresis of periplasmic extracts from R. sphaeroides .

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89 views • 1 slides

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