'Acridine orange' diaporamas de présentation

Practical:2 L ight Microscopy

Practical:2 L ight Microscopy. presented by: Ms Asmaa A Basonbul. Objectives. Recognize different parts of a compound light microscope. Learn how using appropriate objective lenses e.g 10x for focusing 40x for wet preparation 100x oil immersion

567 views • 37 slides

A Novel Approach to Identifying Differential Gene Expression

A Novel Approach to Identifying Differential Gene Expression. Robert J. Pignolo and Oriana Galardi-Este. Methods used to identify differential gene expression. Subtractive cDNA libraries and probes Differential display Microarray analysis 2-D protein electrophoresis.

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Background

288 views • 23 slides

Andrew Rosenzweig, MD Lab Meeting 2.26.08

Development and Validation of an In Vitro Model for Osteoblast Aging: Beta-galactosidase and Acridine Orange. Andrew Rosenzweig, MD Lab Meeting 2.26.08. Goals.

324 views • 15 slides

Acridine orange staining of virus infected host cells to monitor prolif- eration of viralinfection

Acridine orange staining is a methodology for nucleic acid selective metachromatic staining that is useful for following cell cycle determination. The interaction of acridine orange with target molecules (i.e. DNA and RNA) is by intercalation or electrostatic attraction, respectively. This work presents methodology and results of a cell staining that utilizes acri- dine orange fluoresence. It is possible to visualize under dark field ultra- violet microscope the actual viral infected host cells. The color production is stark and easily selected. This method appears to be versatile for a number of various cell types in tissue culture, including human lympho- cytes. This methodology is demonstrated for Epstein Barr Virus (EBV), human immunodeficiency virus (HIV), and laboratory strain of lymphad- enopathy virus (LAV). The results of staining are reproducible and useful for following viral infection and proliferation in tissue culture.

59 views • 5 slides

Acridine orange staining of virus infected host cells to monitor prolif- eration of viralinfection

Acridine orange staining is a methodology for nucleic acid selective metachromatic staining that is useful for following cell cycle determination. The interaction of acridine orange with target molecules (i.e. DNA and RNA) is by intercalation or electrostatic attraction, respectively. This work presents methodology and results of a cell staining that utilizes acri- dine orange fluoresence. It is possible to visualize under dark field ultra- violet microscope the actual viral infected host cells. The color production is stark and easily selected. This method appears to be versatile for a number of various cell types in tissue culture, including human lympho- cytes. This methodology is demonstrated for Epstein Barr Virus (EBV), human immunodeficiency virus (HIV), and laboratory strain of lymphad- enopathy virus (LAV). The results of staining are reproducible and useful for following viral infection and proliferation in tissue culture.

53 views • 5 slides

Acridine orange dimer

Mass Spectrometry Analysis of GoldView. Acridine Orange from Aldrich. Acridine orange dimer. Acridine orange dimer. GoldView. Conclusion. The effective ingredient in GoldView is acridine orange (extremely cheap from Aldrich or Sigma at ~100g/$140!)

287 views • 2 slides