'Affinity purification' diaporamas de présentation

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Biomolecular Interaction: Enzyme + Substrate

CZ5211 Topics in Computational Biology Lecture 6: Biological Pathways I: Molecular Interactions Prof. Chen Yu Zong Tel: 6874-6877 Email: yzchen@cz3.nus.edu.sg http://xin.cz3.nus.edu.sg Room 07-24, level 7, SOC1, NUS. Biomolecular Interaction: Enzyme + Substrate.

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Gene Disruption (cont) & Protein-Protein Interactions October 4, 2006

Gene Disruption (cont) & Protein-Protein Interactions October 4, 2006. Outline Recap Gene Disruption and Drug Discovery RNAi Two Hybrid Affinity Purification Protein Chips. Last Time. 1) Insertional Mutagenesis Transposon Strategies

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Advanced Bioinformatics Lecture 3: Protein-protein interaction

Advanced Bioinformatics Lecture 3: Protein-protein interaction. ZHU FENG zhufeng@cqu.edu.cn http://idrb.cqu.edu.cn/ Innovative Drug Research Centre in CQU. ???????????????. Table of Content. Protein-protein interaction Interaction representations Method A: Two-hybrid assay

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27041, Week 02

27041, Week 02. Review of Week 01. The human genome sequencing project (HGP). Systems Biology and emergent properties. Different model representations. Chen et al., Mol. Biol. Cell., 2004. Model Generation. Systems Biology at a glance. YER001W YBR088C YOL007C YPL127C YNR009W YDR224C

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Protein Complexes in S. cerevisiae and E. coli A Focus on Transcription

Protein Complexes in S. cerevisiae and E. coli A Focus on Transcription. NIH April 7, 2003. Tandem Affinity Purification (TAP) Tagging Strategy for S. cerevisiae. Primer 1. Primer 1. 1. ATG. TAA. Targeted Gene. Targeted Gene. Primer 2. Primer 2. 2. Primer 1. Primer 1. Marker.

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Protein Complexes in S. cerevisiae and E. coli A Focus on Transcription

Protein Complexes in S. cerevisiae and E. coli A Focus on Transcription. NIH April 7, 2003. Tandem Affinity Purification (TAP) Tagging Strategy for S. cerevisiae. Primer 1. Primer 1. 1. ATG. TAA. Targeted Gene. Targeted Gene. Primer 2. Primer 2. 2. Primer 1. Primer 1. Marker.

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TAP(Tandem Affinity Purification)

TAP(Tandem Affinity Purification). Billy Baader Genetics 677. Protein-protein interactions. Protein Identification 20,000+ genes in humans Millions of proteins Understanding human biological processes. Protein Identification. Other available methods -2D gel analysis -Labeling methods

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A highly abbreviated introduction to proteomics

A highly abbreviated introduction to proteomics. A typical shotgun proteomics experiment. Collect tens of thousands of MS/MS spectra Can identify >1,000 proteins from cell lysate. Orbi video: http://apps.thermoscientific.com/media/SID/LSMS/Video/webinar/orbitrap_elite/animation/.

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Advanced Bioinformatics Lecture 1: Introduction to system biology

Advanced Bioinformatics Lecture 1: Introduction to system biology. ZHU FENG zhufeng@cqu.edu.cn http://idrb.cqu.edu.cn/ Innovative Drug Research Centre in CQU. ???????????????. Table of Content. An introduction How to survive What will be covered Signaling pathway Concluding remarks.

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Display of the Viral Epitopes on Lactococcus lactis : A Model for Food Grade Vaccine against EV71

Display of the Viral Epitopes on Lactococcus lactis : A Model for Food Grade Vaccine against EV71. Introduction Material and methods Results. 1. Introduction. Enterovirus 71 infection manifests most frequently as the childhood illness known as hand-foot- and-mouth disease(HFMD).

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Analytical Techniques Utilizing Antibodies : flow cytometry gel electrophoresis

CNBr. ?. Antibodies. Analytical Techniques Utilizing Antibodies : flow cytometry gel electrophoresis immunoprecipitation (IP) immunoblotting microscopy immunofluorescence (IFA) electron microscopy ELISA. antibodies bind proteins with high specificity and affinity

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LUMIER-Assays

LUMIER-Assays. Dr. Manfred Koegl Protein Interaction Unit Genomics And Proteomics Core Facility. LUMIER assays are a reliable, cheap, high-throughput method to measure protein-protein interactions in mammalian cells.

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Serine hydrolase ABPP

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Affinity Ligands for Plasmid DNA Purification YING HAN, GARETH M. FORDE

Affinity Ligands for Plasmid DNA Purification YING HAN, GARETH M. FORDE Chemical Engineering Department, Monash University, Vic 3800, Australia. A. B. Fig. 3: A ribbon computer graphic representation of the three helix bundle of lacI protein (helix-turn-helix-loop-helix). Equilibration.

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B Akiyoshi et al. Nature 468 , 576-579 (2010) doi:10.1038/nature09594

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Affinity beads

Affinity beads: http://www.cd-bioparticles.com/l/Affinity-Magnetic-Particles_20_194_0.html

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