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Investigating Specificity of FP-TAMRA Labeled Urine Using Anti-HIV Antibody Conjugated Beads

This study explores the specificity of affinity purification using FP-TAMRA labeled urine and anti-HIV antibody-conjugated beads. Results show that the beads did not enrich FP-TAMRA labeled bands. Key materials include the FP-TAMRA labeled starting material, eluted protein from the anti-HIV column, and total protein analyses. The evaluation also highlights the use of serine hydrolase activity-based protein profiling (ABPP) and the Sypro Ruby staining method for total protein quantification, providing insights into the efficiency of antibody-based enrichment techniques.

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Investigating Specificity of FP-TAMRA Labeled Urine Using Anti-HIV Antibody Conjugated Beads

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  1. ADDITIONAL FILE 1. Specificity of Affinity purification of FP-TAMRA labeled urine. Beads conjugated with an anti-HIV antibody did not enrich FP-TAMRA labeled bands. 1) FP-TAMRA labeled starting material. 2) Material eluted from anti-HIV column. 3) starting material total protein. 4) Protein eluted from anti-HIV column. Serine hydrolase ABPP Total protein, Sypro Ruby 1 2 1 2

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