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Activity-Based Protein Profiling of Male and Female Urines at Varying Incubation Times

This study explores the Activity-Based Protein Profiling (ABPP) of normal male and female urine samples subjected to different incubation times—5 minutes, 30 minutes, and 90 minutes—at 37°C with pH 9. The focus is on serine hydrolases, utilizing total protein extraction and visualization through Sypro Ruby staining. The findings aim to enhance understanding of protein activity variations in urine, potentially contributing to biomarker discovery and understanding gender-specific metabolic processes.

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Activity-Based Protein Profiling of Male and Female Urines at Varying Incubation Times

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  1. ADDITIONAL FILE 4. Activity based protein profiling (ABPP) of normal male and female urines with different incubation times (5min, 30min, 90min), 37°C, pH 9. Serine hydrolase ABPP Total protein, Sypro Ruby

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