Amino acid analysis - PowerPoint PPT Presentation

F 2006 BIOC 3405

F 2006 BIOC 3405. 09-07-06. Amino Acids. Fundamental particle of proteins Put together into proteins indirectly aa + t-RNA + ATP  aminoacyl-tRNA + H 2 O aminoacyl-tRNA’s  proteins “Residues” – amino acid minus H 2 O. Fischer projection. Change in charge with change in pH. pK a.

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EMF Protein Analysis Flow Chart

Isolated Protein. Gel protein spot (1D / 2D). Blotted on PVDF membrane. Figure I. EMF Protein Analysis Flow Chart. Biological material (Tissue, Cells, Body fluids). HPLC/ Purification. Gel electrophoresis. Digestion/ cleavage. Digestion/ cleavage. Digestion/ cleavage.

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