Autoradiography - PowerPoint PPT Presentation

Vincent A. Chiappinelli, Ph.D. The Ralph E. Loewy Professor

Vincent A. Chiappinelli, Ph.D. The Ralph E. Loewy Professor Chair, Dept. of Pharmacology &amp; Physiology Tel: 202-994-3541 phmvac@gwumc.edu. FUNCTIONAL PROPERTIES OF NEURONAL NICOTINIC RECEPTORS. NIH Competitive Renewal – 21 st Year Starts Dec 2006.

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32 P

32 P. phospho (Ser) PKC Ab. Autoradiography. Rbt475. + Ca 2+ (50 µM). - Ca 2+. IP 3 R1. IP 3 R3. IC 50 ≤ 2 µM. Ca 2+. mass spectrometry: IP 3 R1 and IP 3 R3 performed by Prof. E. Waelkens (Biochemie). Phosphorylated receptor → SDS-PAGE In gel digestion

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Follow-ups from last class

Follow-ups from last class. Autoradiography: How do we know it works?. How do we know the most active neurons are lighting up? Stimulate retina with light Get activity in the right place Amount of activity correlated with intensity of light 2. Electrically stimulate sciatic nerve

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203 views • 5 slides

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