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Investigation of Phosphorylated PKC Receptor by Mass Spectrometry

Study on the phosphorylated PKC receptor by Prof. E. Waelkens using autoradiography and mass spectrometry analysis. Detailed methods include SDS-PAGE, in gel digestion, and peptide extraction and concentration.

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Investigation of Phosphorylated PKC Receptor by Mass Spectrometry

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  1. 32P phospho (Ser) PKC Ab

  2. Autoradiography Rbt475

  3. + Ca2+ (50 µM) - Ca2+ IP3R1 IP3R3 IC50 ≤ 2 µM Ca2+

  4. mass spectrometry: IP3R1 and IP3R3 performed by Prof. E. Waelkens (Biochemie) • Phosphorylated receptor → SDS-PAGE • In gel digestion • Extraction of proteolytic peptides • Concentration of phosphopeptides • Mass spectrometry

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