Investigation of Phosphorylated PKC Receptor by Mass Spectrometry

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Study on the phosphorylated PKC receptor by Prof. E. Waelkens using autoradiography and mass spectrometry analysis. Detailed methods include SDS-PAGE, in gel digestion, and peptide extraction and concentration.

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Jun 22, 2025

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Investigation of Phosphorylated PKC Receptor by Mass Spectrometry

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32P phospho (Ser) PKC Ab
Autoradiography Rbt475

+ Ca2+ (50 µM) - Ca2+ IP3R1 IP3R3 IC50 ≤ 2 µM Ca2+

mass spectrometry: IP3R1 and IP3R3 performed by Prof. E. Waelkens (Biochemie) • Phosphorylated receptor → SDS-PAGE • In gel digestion • Extraction of proteolytic peptides • Concentration of phosphopeptides • Mass spectrometry