'Confocal microscopy' diaporamas de présentation

Confocal Microscopy

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Confocal microscopy vs Second harmonic generation microscopy.

Confocal microscopy vs Second harmonic generation microscopy. ???????? ? B ? ?? : ??? ??? ??? ??? 2012/01/03. ????. A. ??? History Single photon and two photons excitation B. ??? Confocal Microscopy C. ??? Second Harmonic Generation (SHG) microscopy D. ???

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Brightfield microscopy

Brightfield microscopy . Generally only useful for stained biological specimens Unstained cells are virtually invisible. Oblique Illumination. Phase Contrast. http://microscopy.fsu.edu/primer/techniques/ phasegallery/chocells.html. Aberrations. Spherical aberration Most severe

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Microscopy and Surface Analysis 1

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Confocal Microscopy

Confocal Microscopy. M. Chua Cell & Molecular Physiology, UNC Michael Hooker Microscopy Facility BME 2003-04-02. A microscope system which removes out of focus information optically. Consists of a high quality microscope, light source & electronic detector. . Confocal Microscope.

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Announcements

Announcements. 1. Genetics-related course offerings for spring 2003: - BIO 324 Cell biology, 3 credits; 3 hours in lecture - BIO 325 Biotechnology, 3 credits; 5 hours in lab -BIO 397 Seminar in Human gene therapy, 1 credit

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Announcements

Announcements. 1. Genetics-related course offerings for spring 2003: - BIO 324 Cell biology, 3 credits; 3 hours in lecture - BIO 325 Biotechnology, 3 credits; 5 hours in lab -BIO 397 Seminar in Human gene therapy, 1 credit

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Fluorescence microscopy II Advanced approaches

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Chapter 3 Observing Microbes through a Microscope

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Protein-protein interactions

Protein-protein interactions. Masoud Youssefi, MD,PhD Division of microbiology/virology. Introduction. important field in cell biology, biochemistry Localization and trafficking posttranslational modifications signaling networks also important field in viral replication

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1680

1930. 1680. 1960. 1515. Optics. Focus. Dr. Marvin Minsky Confocal microscope: 1957. L aser. S canning. C onfocal M icroscope. LASER. L ight A mplification by the S timulated E mission of R adiation. Dichroic Mirror. Conventional / Confocal. Conventional / Confocal.

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Structured illumination microscopy and reflection confocal

Structured illumination microscopy and reflection confocal. Structured illumination. Sample image through a grating 3X, moving the grating each time by 1/3 pixel Only in-focus data will have sharp fringes Out-of-focus data will be relatively invariant

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500 nm

ASSESSING THE EFFECT OF BIOLOGICAL AGENT INGESTION A SYSTEMS BIOLOGY BIOLOGICAL AGENT MODELING PLATFORM DR. AMANDA N. PAYNE, MICROBIOLOGIST, NAVAL SURFACE WARFARE CENTER-DAHLGREN. EPITHELIAL CELL MODEL. IN VITRO GUT MODEL. ZIHLER ET AL. 2011. GUT MICROBES. BACILLUS ANTHRACIS. 500 nm.

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Center for Biologics Evaluation and Research FDA

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Basics of Image Acquisition

Basics of Image Acquisition. Elvira Garcia Osuna Tad Merryman ITR Meeting 7 October 2004. Cell Types. HeLa Cells from Henrietta Lacks Died in 1951 of cervical cancer 3T3 Cells from mouse embryo. Cells are mostly water.

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Computationally Efficient Focal Field Calculation

Computationally Efficient Focal Field Calculation. Jie Yee Chan (Mentee) Santosh Tripathi (Mentor). Importance of Focused Laser Beam. Nanotechnology Confocal Microscopy Optical Data Storage Manipulation of Microscopic Particles Optical Tweezers. Focal Field. Final Objective.

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The visual and refractive results of patients treated with PRK and LASEK: A paired eye study

The visual and refractive results of patients treated with PRK and LASEK: A paired eye study. Volkan Hurmeric , MD Uzeyir Erdem, MD Selim Dagli, MD Gulhane Military Medical Academy Ophthalmology Department Ankara-Turkey.

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? v ? 3 (FITC) and F-actin (TR)

Supplemental figure. A. Wt b 3-2 b 3-3 b 3-4 b 3-5 b 3-6 mock mock mock mock. B. NP-9 ? 3 -3. NP-9 ? 3 -4. NP-9 ? 3 -5. NP-9 ? 3 -6. ? v ? 3 (FITC) and F-actin (TR). Supplemental figure. NP-9 ? 3 clones: expression of ? 3 and cell morphology.

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G van den Bogaart et al . Nature 000 , 1 - 4 (2011) doi:10.1038/nature10545

Confocal microscopy of syntaxin-1A domains in artificial membranes. G van den Bogaart et al . Nature 000 , 1 - 4 (2011) doi:10.1038/nature10545.

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