'Contigs' diaporamas de présentation

DNA Sequencing

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Whole Genome Assembly

Whole Genome Assembly. Mate-pairs allow you to merge islands (contigs) into super-contigs. Mate Pairs. Make clones of truly predictable length. EX: 3 sets can be used: 2Kb, 10Kb and 50Kb. The variance in these lengths should be small.

543 views • 45 slides

Large-Scale Global Alignments Multiple Alignments

Large-Scale Global Alignments Multiple Alignments. Lecture 10, Thursday May 1, 2003. ARACHNE: Steps to Assemble a Genome. 1. Find overlapping reads. 2. Merge good pairs of reads into longer contigs. 3. Link contigs to form supercontigs. 4. Derive consensus sequence.

435 views • 33 slides

DNA Sequencing

733 views • 32 slides

Members: Eishita Tyagi Sandeep Namburi Aarthi Talla Vinay Vyas Amin Momin Jay Humphrey

Members: Eishita Tyagi Sandeep Namburi Aarthi Talla Vinay Vyas Amin Momin Jay Humphrey. COMPUTATIONAL GENOMICS GENOME ASSEMBLY. Contents. Assembly De novo Algorithms Involved Reference Assembly problems Task and Strategy. How do we get Reads?. Assembly.

286 views • 28 slides

Members: Eishita Tyagi Sandeep Namburi Aarthi Talla Vinay Vyas Amin Momin Jay Humphrey

Members: Eishita Tyagi Sandeep Namburi Aarthi Talla Vinay Vyas Amin Momin Jay Humphrey. COMPUTATIONAL GENOMICS GENOME ASSEMBLY. Contents. Assembly De novo Algorithms Involved Reference Assembly problems Task and Strategy. How do we get Reads?. De novo Assembly. Reads.

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Solanum lycopersicum Chromosome 4 Sequencing Update

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Sequencing and Assembly

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The Genome Assemblies of Tasmanian Devil

The Genome Assemblies of Tasmanian Devil. Zemin Ning The Wellcome Trust Sanger Institute. Outline of the Talk:. The Phusion2 pipeline Flow-sorting sequencing Assigning contigs to individual chromosomes The Devil genome assembly Future work. Phusion2 Assembly Pipeline. Assembly.

342 views • 16 slides

Assembling the Glanville fritillary genome

CSC Conference 2.6.2010 Next generation sequencing data analysis. Assembling the Glanville fritillary genome. Panu Somervuo University of Helsinki MRG group & DNA sequencing and genomics lab. Next generation sequencing. Roche 454 Illumina Solexa ABI SOLiD. Assembly pipeline. Newbler

265 views • 13 slides

Human Genome Project

Human Genome Project. Basic Strategy. How to determine the sequence of the roughly 3 billion base pairs of the human genome. Started in 1995. Various side projects: genetic diseases, variations between individuals, ethnic variation, comparison to other species. Strategy:

515 views • 12 slides

Theobroma cacao Integrated Physical and Genetic Map

Theobroma cacao Integrated Physical and Genetic Map. 2 BAC Libraries 250 Genetic Markers. Quality Statistics (Both BAC Libs ). Number of BACs Fingerprinted: 72,192 Number of BACs pass Q/C: 67,408 (93.3%) Criteria: GeneMapper - Sizing of fragments in comparison so size std.

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Sequence Assembly of Medicago Truncatula Chromosomes

Selecting New BAC clones to be sequenced. Selecting New BAC clones to be sequenced. Assembly Pipeline. Conclusion. Clone Selection Efficiency. BAC sequences assembled by PhredPhrap.

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(a)

(c). (b). (a). Additional file 9: Venn diagram of contigs obtained after BLASTX in NCBI-NR,TAIR and BLASTN for Cotton EST database. Contigs of (a) Drought tolerant genotype (GujCot-21) (b) Sensitive genotype (RAHS-IPS-187) (c) Super contigs.

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100

4000. 3500. 3000. 600. Number of Contigs. 500. 400. 300. 200. 100. Number of ESTs. 0. 2-5. 482. 828. 6-10. 11-15. 16-20. 21-25. 26-30. 31-35. 36-40. 41-45. 46-50. 51-55. 56-60. 61-65. 66-70. 71-75. 76-80. 81-85. 86-90. 91-95. 96-100. 101-200. 201-300. 301-400.

78 views • 1 slides