'Counter stain' diaporamas de présentation

Microscopy and Cell Structure

Microscopy and Cell Structure. Chapter 3. Microscope Techniques Microscopes. Microscopes Most important tool for studying microorganisms Use viable light to observe objects Magnify images approximately 1,000x Electron microscope, introduced in 1931, can magnify images in excess of 100,000x

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Gram staining Techniques

Gram staining Techniques. Some history. Bacteria are translucent Staining make them visible under microscope 1884 Hans Christian Gram ? stained cells and found that some lost their color when excess stain was washed off Differential stain ? distinction between 2 types of bacteria.

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PHT 226 Lab # 3

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PHT 226 Lab # 3

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Microbes and Society

Microbes and Society. Brief Introduction to Microbes. Prokaryotes vs. Eukaryotes. Prokaryotes have no membrane bound nucleus or organelles. They have a single closed loop of DNA. Most of the prokaryotes reproduce by fission.

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Natural stain : too expensive. Synthetic stain :

Staining: Bacteria are too small, colorless( Transperant ).Therefore they must be stained to study :morphology, size, shape, arrangment of microorganisms. Types of bacterial stain ( origin) :. Natural stain : too expensive. Synthetic stain :

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EXPERIMENT # 3

EXPERIMENT # 3. SMEAR PREPARATION SIMPLE STAIN GRAM STAIN. Work in groups of two. . epidermidis. Dispose extra stain inside the plastic bottle in the sinks. 2. +. +. +. _. _ _. _ _. _ _. _ _. SIMPLE STAIN (w/ Methylene blue). Work in groups of two.

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Ex. 9 Acid-Fast Stain

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Lab Exercise 7 :

Lab Exercise 7 :. Acid Fast Staining. Nonacid Fast. Kinyoun Acid-Fast Staining Procedure 1. A sample of cells is mixed with a drop of water on a clean slide to make a smear. After air drying, the slide is heat fixed.

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